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Acute transcriptomic changes in murine RAW 264.7 cells following pseudorabies virus infection

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Abstract

Next-generation sequencing enables the evaluation of gene expression changes resulting from virus–host interactions at the RNA level. Pseudorabies virus (PRV) causes substantial economic loss in the swine industry. Recent research has revealed that PRV can be transmitted to and infect humans as well. To identify physiopathological and pathological responses post-PRV infection, we characterized transcriptomic changes in the murine RAW 264.7 cell line over the course of 36 h. In total, 156, 153, and 190 differentially expressed genes were identified at 2 h, 12 h, and 36 h, respectively. Seven differentially expressed genes (Trim27, Ccdc117, Mrps12, Ccl4, Cerkl, Ubald1, and Hmga1-rs1) were present across all treatment groups. Our findings expand our knowledge of gene regulation and immune response following PRV infection. These differentially expressed genes can subsequently improve our understanding of PRV pathogenesis.

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Data availability

The sequence data of this study have been deposited in the Sequence Read Archive (http://www.ncbi.nlm.nih.gov/sra, accession number PRJNA689234). All datasets generated in this study are included in the article/Supplementary Material.

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Acknowledgements

The authors would like to acknowledge Fang Liu for her outstanding help with data analysis.

Funding

This work was supported by grants from the Natural Science Foundation of Henan Province (202300410213).

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    Authors and Affiliations

    1. College of Veterinary Medicine, Henan Agricultural University, 450000, Zhengzhou, Henan province, China

      Chao Tong, Peng-Fei Fu, Sheng-Li Ming, Lei Zeng, He-Shui Zhu & Jiang Wang

    2. Wuhu Overseas Student Pioneer Park, 241006, Wuhu, China

      Chao Tong

    3. Key Laboratory of Animal Biochemistry and Nutrition, Henan Agricultural University, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, 450046, Zhengzhou, Henan Province, China

      Peng-Fei Fu, Sheng-Li Ming, Lei Zeng, He-Shui Zhu & Jiang Wang

    4. Key Laboratory of Animal Growth and Development, The Education Department of Henan Province, 450046, Zhengzhou, Henan Province, China

      Peng-Fei Fu, Sheng-Li Ming, Lei Zeng, He-Shui Zhu & Jiang Wang

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    1. Chao Tong
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    Contributions

    Chao Tong, He-Shui Zhu and Jiang Wang designed the study, drafted the manuscript, and revised the manuscript. Peng-Fei Fu and Sheng-Li Ming collected the samples and performed the experiments. Peng-Fei Fu, Lei Zeng, and He-Shui Zhu analyzed the data. All authors read and approved of the final version of the manuscript that was submitted for publication.

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    Correspondence to He-Shui Zhu or Jiang Wang.

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    The authors have no relevant financial or non-financial interests to disclose.

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    No animal work was performed for this study.

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    Handling Editor: Zhongjie Shi

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    Chao Tong and Peng-Fei Fu authors contributed equally to this work.

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    Tong, C., Fu, PF., Ming, SL. et al. Acute transcriptomic changes in murine RAW 264.7 cells following pseudorabies virus infection. Arch Virol 167, 2623–2631 (2022). https://doi.org/10.1007/s00705-022-05598-1

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    • DOI: https://doi.org/10.1007/s00705-022-05598-1

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