Abstract
Viral metagenomics has been used in numerous animal virus discoveries. Recently, an unprecedented diversity of CRESS DNA viruses was identified using this method, and this has expanded our understanding of the environmental distribution and host range of CRESS DNA viruses. In this study, using an unbiased viral metagenomics approach, we investigated the fecal virome of chickens collected from two farms of Anhui Province, China. Five novel CRESS DNA viruses were obtained and characterized. The genome of the five viruses is 2,401-2,742 bp in length, containing two ORFs in the same orientation. Phylogenetic analysis indicated that all five viruses have a closer genetic relationship to smacoviruses than to other viruses in the order Cremevirales. Pairwise comparison of Rep amino acid sequences showed that these five viruses had only low amino acid sequence identity (8.9%-30.6%) to members of the family Smacoviridae, and the sequence identity among the five smaco-like viruses and other unclassified smacovirus strains was 70.3–95.8%. These findings broaden our knowledge of the genetic diversity of CRESS DNA viruses and provide a basis for classification of unclassified smacoviruses.
References
Tisza MJ, Pastrana DV, Welch NL et al (2020) Discovery of several thousand highly diverse circular DNA viruses. Elife 9. https://doi.org/10.7554/eLife.51971
Krupovic M, Varsani A, Kazlauskas D et al (2020) Cressdnaviricota: a Virus Phylum Unifying Seven Families of Rep-Encoding Viruses with Single-Stranded, Circular DNA Genomes.J Virol94
Zhao L, Rosario K, Breitbart M, Duffy S (2019) Eukaryotic Circular Rep-Encoding Single-Stranded DNA (CRESS DNA) Viruses: Ubiquitous Viruses With Small Genomes and a Diverse Host Range. Adv Virus Res 103:71–133
Krupovic M, Varsani A (2021) A 2021 taxonomy update for the family Smacoviridae. Arch Virol 166:3245–3253
Anindita PD, Sasaki M, Gonzalez G et al (2019) Discovery and genetic characterization of diverse smacoviruses in Zambian non-human primates. Sci Rep 9:5045
Steel O, Kraberger S, Sikorski A et al (2016) Circular replication-associated protein encoding DNA viruses identified in the faecal matter of various animals in New Zealand. Infect Genet Evol 43:151–164
Fontenele RS, Lacorte C, Lamas NS et al (2019) Single Stranded DNA Viruses Associated with Capybara Faeces Sampled in Brazil.Viruses11
Tochetto C, Muterle Varela AP, Alves de Lima D et al (2020) Viral DNA genomes in sera of farrowing sows with or without stillbirths. PLoS ONE 15:e0230714
Wang H, Li S, Mahmood A et al (2018) Plasma virome of cattle from forest region revealed diverse small circular ssDNA viral genomes. Virol J 15:11
Yang S, He Y, Chen X et al (2021) Viral Metagenomics Reveals Diverse Viruses in the Feces Samples of Raccoon Dogs. Front Vet Sci 8:693564
Chrzastek K, Kraberger S, Schmidlin K et al (2021) Diverse Single-Stranded DNA Viruses Identified in Chicken Buccal Swabs.Microorganisms9
Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3415
Rosario K, Duffy S, Breitbart M (2012) A field guide to eukaryotic circular single-stranded DNA viruses: insights gained from metagenomics. Arch Virol 157:1851–1871
Ng TFF, Zhang W, Sachsenröder J et al (2015) A diverse group of small circular ssDNA viral genomes in human and non-human primate stools. Virus Evol 1:vev017
Kaszab E, Marton S, Forró B et al (2018) Characterization of the genomic sequence of a novel CRESS DNA virus identified in Eurasian jay (Garrulus glandarius). Arch Virol 163:285–289
Fontes EP, Gladfelter HJ, Schaffer RL et al (1994) Geminivirus replication origins have a modular organization. Plant Cell 6:405–416
Stenger DC, Revington GN, Stevenson MC, Bisaro DM (1991) Replicational release of geminivirus genomes from tandemly repeated copies: evidence for rolling-circle replication of a plant viral DNA. Proc Natl Acad Sci U S A 88:8029–8033
Funding
This work was supported by the National Key Research and Development Programs of China (No. 2017YFC1200201), Jiangsu Provincial Key Research and Development Projects (No. BE2017693), the Independent Project of Chengdu Research Base of Giant Panda Breeding (No. 2020CPB-C11), and the Shandong Natural Resources Fund (No. ZR2019MC036).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
The authors declare that they have no competing interests.
Ethical approval
The research reported here did not involve experimentation with human participants or animals.
Additional information
Handling Editor Ana Cristina Bratanich
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Shixing Yang, Dianqi Zhang and Yuyang Zhang contributed equally to this work.
Electronic Supplementary Material
Below is the link to the electronic supplementary material
Rights and permissions
About this article
Cite this article
Yang, S., Zhang, D., Zhang, Y. et al. Multiple novel smaco-like viruses identified in chicken cloaca swabs. Arch Virol 167, 2703–2708 (2022). https://doi.org/10.1007/s00705-022-05577-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-022-05577-6