Abstract
A putative new emaravirus, named “ailanthus crinkle leaf-associated emaravirus” (ACrLaV), was detected in Ailanthus altissima with severe crinkle symptoms by RNA-Seq and RT-PCR. Four viral segments associated with ACrLaV were identified and fully sequenced, except for a few nucleotides at the genomic termini. The RNA-dependent RNA polymerase (RNA1), glycoprotein (RNA2), nucleocapsid protein (RNA3), and movement protein (RNA4), showed 26.5%-57%, 17%-49.9%, 14.4%-40.4%, and 14.1%-65.9% amino acid sequence identity, respectively, to those of known emaraviruses. All four ACrLaV genomic RNA segments are most closely related to those of common oak ringspot-associated virus from Germany, as supported by sequence comparisons and phylogenetic analysis. ACrLaV is considered a distinct member of the genus Emaravirus, and this is the first report of an emaravirus in A. altissima.
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Acknowledgements
We would like to thank Dr. Zhenguo Du of Fujian Agriculture and Forestry University for useful discussion on the genomic constitution.
Funding
This work was supported by funds from the Natural Science Foundation of Liaoning Province (2021-MS-341) and the Program for Young and Middle-Aged Scientific and Technological Innovation Talents of Shenyang City (RC210161).
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An, W., Li, C., Zhang, S. et al. A putative new emaravirus isolated from Ailanthus altissima (Mill.) Swingle with severe crinkle symptoms in China. Arch Virol 167, 2403–2405 (2022). https://doi.org/10.1007/s00705-022-05569-6
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DOI: https://doi.org/10.1007/s00705-022-05569-6