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Complete genome analysis of a novel chuvirus from a southern green stink bug (Nezara viridula)

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Abstract

A novel chuvirus from a southern green stink bug (Nezara viridula) was identified by RNA sequencing in this study and was tentatively named “Ningbo southern green stink bug chuvirus 1” (NBSGSBV-1). The complete genome sequence of NBSGSBV-1 consists of 11,375 nucleotides, and the genome was found to be circular by ‘around-the-genome’ reverse transcription polymerase chain reaction (RT-PCR) and Sanger sequencing. Three open reading frames (ORFs) were predicted in the NBSGSBV-1 genome, encoding a large polymerase protein (L protein), a glycoprotein (G protein), and a nucleocapsid protein (N protein). A phylogenetic tree was constructed based on all of the currently available RNA-dependent RNA polymerase amino acid sequences of viruses of the family Chuviridae, and NBSGSBV-1 was found to cluster together with Sanya chuvirus 2 and Hubei odonate virus 11, indicating that NBSGSBV-1 might belong to the genus Odonatavirus. Five conserved sites were identified in the L proteins of NBSGSBV-1 and other chuviruses. The abundance and characteristics of the NBSGSBV-1-derived small interfering RNAs suggested that NBSGSBV-1 actively replicates in the host insect. To the best of our knowledge, this is the first report of a chuvirus identified in a member of the insect family Pentatomidae. The discovery and characterization of NBSGSBV-1 will help us to understand the diversity of chuviruses in insects.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (U20A2036) and Ningbo Science and Technology Innovation 2025 Major Project (2019B10004).

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Authors and Affiliations

  1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China

    Peng-Peng Ren, Zhuang-Xin Ye, Sai-Nan Wang, Jun-Min Li, Jian-Ping Chen, Chuan-Xi Zhang & Jia-Bao Lu

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  1. Peng-Peng Ren
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Correspondence to Jia-Bao Lu.

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Supplementary Information

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Supplementary Fig. S1 Sanger sequencing of the conjunction region of the 5′ and 3′ termini of NBSGSBV-1

705_2022_5560_MOESM2_ESM.tif

Supplementary Fig. S2 Multiple sequence alignment and locations of motifs in the L proteins of NBSGSBV-1 and closely related chuviruses. Five conserved motifs were identified using MEME, and the conserved sites are indicated by red boxes

Supplementary file3 (PDF 190 KB)

Supplementary file4 (PDF 103 KB)

Supplementary File S1 The COI sequence of Nezara viridula

Supplementary File S2 The full genome sequence of NBSGSBV-1

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Ren, PP., Ye, ZX., Wang, SN. et al. Complete genome analysis of a novel chuvirus from a southern green stink bug (Nezara viridula). Arch Virol 167, 2423–2427 (2022). https://doi.org/10.1007/s00705-022-05560-1

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  • DOI: https://doi.org/10.1007/s00705-022-05560-1

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