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First detection of Lake Sinai virus in the Czech Republic: a potential member of a new species

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Abstract

Lake Sinai virus (LSV) is one of over 20 honey bee viruses. Variants of LSV have been classified as members of two officially recognised species, Lake Sinai virus 1 and Lake Sinai virus 2. However, there are currently a limited number of whole-genome sequences, and the genetic variability of the virus indicates that additional species may need to be established. Extracted nucleic acid of 209 honey bee samples was screened by PCR for 11 honey bee viruses. LSV was the third most abundant virus (36.9% of positive samples), after Apis mellifera filamentous virus (72.2%) and deformed wing virus (52.5%). LSV-positive samples were analyzed further by PCR with primers targeting the region encoding the viral RNA-dependent RNA polymerase. Subsequently, the PCR products were sequenced, and the resulting sequences were used for a first round of phylogenetic analysis. Based on those results, several isolates were selected for whole-genome sequencing, and the complete genome sequences were used for additional phylogenetic analysis. The results indicated the presence of at least three genetically distinct groups of LSV in the Czech Republic, the most prevalent one being related to LSV 2 but too dissimilar to be considered a member of the same species. Two sequences of a major LSV strain cluster native to the Czech Republic were determined, representing the first Czech LSV strains published to date.

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Data availability

The GenBank accession numbers for the LSV sequences reported in this paper are MZ773494, MZ773495, and OK245389-OK245413, as specified in a supplementary table (Online Resource 2).

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Acknowledgments

The authors would like to thank Ing. Dalibor Titěra, CSc from the Bee Research Institute in Dol for kindly providing honey bee samples.

Funding

This research was funded by the Ministry of Agriculture of the Czech Republic, Grant number QK1910286.

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Authors and Affiliations

  1. Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic

    Eliška Čukanová, Romana Moutelíková & Jana Prodělalová

  2. Department of Diseases of Zoo Animals, Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic

    Eliška Čukanová

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Contributions

The experiments described in this study were conceived and designed by JP and RM. The diagnostic testing was performed by EČ and JP. Sequencing, genotyping, data analysis, and writing of the manuscript were carried out by EČ and RM. All authors reviewed the manuscript and approved the final version. JP acquired funding and administered the project.

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Correspondence to Eliška Čukanová.

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Čukanová, E., Moutelíková, R. & Prodělalová, J. First detection of Lake Sinai virus in the Czech Republic: a potential member of a new species. Arch Virol 167, 2213–2222 (2022). https://doi.org/10.1007/s00705-022-05548-x

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