Abstract
Carrot virome analysis using high-throughput sequencing revealed the presence of two RNA molecules with properties of satellite RNAs that are homologous to the satellite RNA of cereal yellow dwarf virus-RPV (CYDV-RPV). Satellite 1 is 298 nt long, while satellite 2 is 368 nt long. Their positive and negative genome strands contain hammerhead ribozymes similar to those found in other self-cleaving satellite RNAs. While both satellites were detected in Spanish carrot populations, only satellite 2 was found in French carrot populations. The most likely helper virus for these two satellites is carrot red leaf virus (CtRLV), which, like CYDV-RPV, is a polerovirus.
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Data availability
The genome sequences for the hammerhead satellites reported here have been deposited in the GenBank database. The raw sequence datasets are available on request from the authors.
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Acknowledgements
The authors would like to thank the Platform GetPlaGe (INRAE, Toulouse, France) for the Illumina sequencing of the French samples.
Funding
This study was funded by the European Union through the Horizon 2020 Marie Skłodowska-Curie Actions Innovative Training Network (H2020 MSCA-60 ITN) project “INEXTVIR” (GA 813542).
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Babalola, B.M., Schönegger, D., Faure, C. et al. Identification of two novel putative satellite RNAs with hammerhead structures in the virome of French and Spanish carrot samples. Arch Virol 167, 2287–2292 (2022). https://doi.org/10.1007/s00705-022-05538-z
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DOI: https://doi.org/10.1007/s00705-022-05538-z