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Complete genome sequence analysis of Paris alphapartitivirus 1: a novel member of the genus Alphapartitivirus infecting Paris polyphylla var. yunnanensis

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Abstract

A novel double-stranded RNA (dsRNA) virus, tentatively named “Paris alphapartitivirus 1” (ParAPV1, OL960006-OL960007), was detected in Paris polyphylla var. yunnanensis plants exhibiting leaf chlorosis and shrinkage symptoms in Yunnan. Its complete genome sequence was determined using Illumina and Sanger sequencing. ParAPV1 has a bipartite genome that consists of dsRNA1 (1,917 bp) encoding the viral RNA-dependent RNA polymerase (RdRp), and dsRNA2 (1,818 bp) encoding the putative coat protein (CP). Sequence comparisons showed that the RdRp and CP of ParAPV1 are most similar to those of pear alphapartitivirus (PpPV2), with 69.97% and 54.21% amino acid sequence identities respectively. Phylogenetic analysis of the RdRp amino acid sequences of ParAPV1 and other partitiviruses showed that ParAPV1 cluster with viruses in a clade containing alphapartitiviruses, and that its closest known relatives are PpPV2 (BBA66577) and rose partitivirus (RoPV, ANQ45203S). Taken together, these results suggest that ParAPV1 should be regarded as a new member of genus Alphapartitivirus in the family Partitiviridae. This is the first report of a partitivirus infecting P. polyphylla var. yunnanensis.

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Data availability

The dataset generated during the current study is available in the GenBank database (accession no. OL960006 and OL960007).

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Funding

This study was funded by the National Natural Science Foundation of China (grant number 81860774).

Author information

Author notes

  1. Zhe Wang and Rex Frimpong Anane equally contributed to this study.

Authors and Affiliations

  1. Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Zhe Wang, Zeli Chen, Like Gao, Shangyun Li, Bifan Chu, Guosong Wen & Mingfu Zhao

  2. Research and Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, 32 Jiaochang Donglu, Kunming, 650201, Yunnan, China

    Zhe Wang, Zeli Chen, Like Gao, Shangyun Li, Bifan Chu, Guosong Wen & Mingfu Zhao

  3. Monash Biomedicine Discovery Institute, and Department of Biochemistry and Molecular Biology, Monash University, 23 Innovation Walk, Clayton, VIC, 3800, Australia

    Rex Frimpong Anane

  4. College of Agronomy and Biotechnology, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Zeli Chen, Shangyun Li & Guosong Wen

Authors
  1. Zhe Wang
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  2. Rex Frimpong Anane
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  3. Zeli Chen
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  4. Like Gao
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  5. Shangyun Li
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  7. Guosong Wen
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  8. Mingfu Zhao
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Corresponding authors

Correspondence to Guosong Wen or Mingfu Zhao.

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Conflict of interest

Author Zhe Wang declares that she has no conflict of interest. Author Rex Frimpong Anane declares that he has no conflict of interest. Author Zeli Chen declares that she has no conflict of interest. Author Like Gao declares that she has no conflict of interest. Author Shangyun Li declares that he has no conflict of interest. Author Bifan Chu declares that he has no conflict of interest. Author Guosong Wen declares that he has no conflict of interest. Author Mingfu Zhao declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Wang, Z., Anane, R.F., Chen, Z. et al. Complete genome sequence analysis of Paris alphapartitivirus 1: a novel member of the genus Alphapartitivirus infecting Paris polyphylla var. yunnanensis. Arch Virol 167, 2365–2370 (2022). https://doi.org/10.1007/s00705-022-05531-6

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  • DOI: https://doi.org/10.1007/s00705-022-05531-6

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