Abstract
The transmembrane zinc metalloprotease ZMPSTE24 works in cooperation with interferon-induced transmembrane protein 3 (IFITM3) to restrict entry of several enveloped viruses. We investigated the role of ZMPSTE24 in porcine reproductive and respiratory syndrome virus (PRRSV) replication. ZMPSTE24 overexpression significantly reduced PRRSV replication in MARC-145 cells. Interestingly, knockdown of endogenous ZMPSTE24 did not significantly impact virus replication. There was no significant difference in the percentage of PRRSV-positive cells and viral RNA copies at 3 hours postinfection (hpi) between cells transfected with ZMPSTE24-FLAG and the vector control. Our results suggest that ZMPSTE24 overexpression may restrict PRRSV replication at a post-entry step.
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Funding
This study was partly supported by Animal Health and Production and Animal Products: Animal Health and Disease (Grant no. 2021-67016-34460/project accession no. 1025717) from the USDA National Institute of Food and Agriculture (NIFA), and Hatch (SD00H660-19) and Hatch Multi State (SD00R656-16) from USDA NIFA and South Dakota Agricultural Experiment Station.
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XW designed the study, analyzed the data, and wrote the manuscript. PK conducted experiments, analyzed the results, and wrote the manuscript. SA conducted experiments and analyzed data. EN and SL provided reagents/analytic tools. MH contributed to confocal microscopy and analysis.
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Katwal, P., Aftab, S., Nelson, E. et al. Role of zinc metalloprotease (ZMPSTE24) in porcine reproductive and respiratory syndrome virus (PRRSV) replication in vitro. Arch Virol 167, 2281–2286 (2022). https://doi.org/10.1007/s00705-022-05529-0
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DOI: https://doi.org/10.1007/s00705-022-05529-0