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Characterization of a novel magoulivirus isolated from the phytopathogenic fungus Verticillium dahlia

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Abstract

A new positive-sense single-stranded RNA (+ssRNA) mycovirus, Verticillium dahliae magoulivirus 1 (VdMoV1), was isolated from two strains (2-19 and XLZ70) of Verticillium dahliae. The complete genome of VdMoV1 is 2303 nucleotides (nt) in length and has a large open reading frame (nt positions from 61 to 1938) encoding an RNA-dependent RNA polymerase (RdRp). A multiple sequence alignment indicated that the central region of the RdRp encoded by VdMoV1 contains eight typical viral RdRp motifs. BLASTp analysis demonstrated that VdMoV1 has the highest sequence identity (86.88%) to Bremia lactucae associated ourmia-like virus 2 (BlaOLV2). Phylogenetic analysis revealed that VdMoV1 is a new member of the genus Magoulivirus. As far as we know, VdMoV1 is the first reported member of the family Botourmiaviridae infecting V. dahliae.

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Acknowledgements

This research was funded by Scientific Research Foundation of the Higher Education Institutions of Hubei Province, China (S202110504009) and the National Natural Science Foundation of China (32072475).

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Authors and Affiliations

  1. College of Life Science, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, 843300, China

    Jiamin Gao & Lili Zhang

  2. State Key Laboratory of Agricultural Microbiology, The Provincial Key Lab of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Jiamin Gao, Nian Wang, Jiatao Xie & Daohong Jiang

  3. Department of Plant Protection, Shanxi Agricultural University, Taigu, 030801, China

    Jichun Jia

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  1. Jiamin Gao
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  2. Nian Wang
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Correspondence to Lili Zhang.

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Gao, J., Wang, N., Jia, J. et al. Characterization of a novel magoulivirus isolated from the phytopathogenic fungus Verticillium dahlia. Arch Virol 167, 2387–2390 (2022). https://doi.org/10.1007/s00705-022-05524-5

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  • DOI: https://doi.org/10.1007/s00705-022-05524-5

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