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A novel nepovirus causing a chlorotic fleck disease on common bean (Phaseolus vulgaris L.)

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Abstract

Two common bean leaf samples from Ethiopia that had shown chlorotic fleck and veinal mosaic symptoms but tested ELISA-negative for known viruses were mechanically transmitted to herbaceous hosts to obtain virus isolates ET-773/4 and ET-779. Virus purification from Chenopodium quinoa systemically infected with ET-773/4 yielded icosahedral particles measuring ~ 30 nm in diameter and containing a single capsid protein of ~ 58 kDa, suggesting a nepovirus infection. Analysis of nucleotide sequences generated from RNA1 and RNA2 of the isolates indicated that they represent a distinct virus species in the genus Nepovirus. Surprisingly, the most closely related sequence in the GenBank database was that of Hobart nepovirus 3, an incompletely described metagenomic sequence obtained from honey bees in Tasmania. This new nepovirus from Ethiopia is provisionally named "bean chlorotic fleck virus".

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Data availability

The sequence data generated for this study can be found in the NCBI database (accession numbers ON148341-ON148344).

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Acknowledgements

This work was supported partly by a grant of the German Federal Ministry for Economic Cooperation to HJV and by a scholarship from the Alexander von Humboldt foundation to AA. We thank Angelika Sieg-Müller for excellent technical assistance and D.-E. Lesemann for electron microscopy.

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Authors and Affiliations

  1. Department of Biological Sciences and Biotechnology, Botswana International University of Science and Technology, Private Bag 16, Palapye, Botswana

    Adane Abraham

  2. Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa

    Dawit B. Kidanemariam

  3. Plant Virus Department, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Culture, Braunschweig, Germany

    Stephan Winter

  4. Im Spargelfeld 1, 38162, Cremlingen, Germany

    H. J. Vetten

Authors
  1. Adane Abraham
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  2. Dawit B. Kidanemariam
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  3. Stephan Winter
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  4. H. J. Vetten
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Contributions

Conception and design: AA, HJV, SW. Experiment: AA and HJV. Data analysis: AA, DBK, and HJV. Draft writing: AA and HJV. Review and editing: AA, HJV, SW, and DBK. All authors have read and agreed to the published version of the manuscript.

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Conflict of interest

The authors declare no conflicts of interest.

Ethical approval

The present study followed international, national, and/or institutional guidelines for the care and use of experimental animals (here: rabbits for antibody production) and complied with relevant German legislation at Julius Kuehn Institute at the time of the study.

Additional information

Communicated by Stephen John Wylie.

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Abraham, A., Kidanemariam, D.B., Winter, S. et al. A novel nepovirus causing a chlorotic fleck disease on common bean (Phaseolus vulgaris L.). Arch Virol 167, 2057–2061 (2022). https://doi.org/10.1007/s00705-022-05507-6

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  • DOI: https://doi.org/10.1007/s00705-022-05507-6

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