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Molecular characterization of a novel botourmiavirus with inverted complementary termini from the rice blast fungus Magnaporthe oryzae isolate HF04

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Abstract

A novel positive-sense single-stranded RNA mycovirus, designated as "Magnaporthe oryzae botourmiavirus 10" (MoBV10), was identified in the rice blast fungus Magnaporthe oryzae isolate HF04. MoBV10 has a single genomic RNA segment consisting of 2,448 nucleotides, which contains a single open reading frame encoding an RNA-dependent RNA polymerase. Genome comparison and phylogenetic analysis indicated that MoBV10 is a new member of the genus Betascleroulivirus in the family Botourmiaviridae. The 5’- and 3’-terminal sequences of the genomic RNA of MoBV10 have inverted complementarity and potentially form a panhandle structure, which is very rare in RNA viruses.

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Funding

This research was funded by the National Natural Science Foundation of China (NFSC no. 31201474).

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Authors and Affiliations

  1. College of Agriculture, Yangtze University, Jingzhou, 434005, Hubei, China

    Simnin Shuai, Hong Zheng, Hang Ding, Yao Wang, Jinzhe Li, Fuyu Liu, Fengying Liu, Hongliu An, Shouguo Fang, Songbai Zhang & Qingchao Deng

  2. Hubei Engineering Research Center for Pest Forewarning and Management, Jingzhou, China

    Qingchao Deng

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  1. Simnin Shuai
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  2. Hong Zheng
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  3. Hang Ding
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  4. Yao Wang
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  7. Fengying Liu
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  11. Qingchao Deng
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Correspondence to Qingchao Deng.

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Shuai, S., Zheng, H., Ding, H. et al. Molecular characterization of a novel botourmiavirus with inverted complementary termini from the rice blast fungus Magnaporthe oryzae isolate HF04. Arch Virol 167, 1899–1903 (2022). https://doi.org/10.1007/s00705-022-05506-7

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  • DOI: https://doi.org/10.1007/s00705-022-05506-7

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