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Complete genome analysis of a novel iflavirus from the spotted lanternfly Lycorma delicatula

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Abstract

The spotted lanternfly (Lycorma delicatula) is an invasive pest that causes serious economic losses in fruit and wood production. Here, we identified a novel iflavirus named "Lycorma delicatula iflavirus 1" (LDIV1), in a spotted lanternfly. The full genome sequence of LDIV1 is 10,222 nt in length and encodes a polyprotein containing a picornavirus capsid-protein-domain-like domain, a cricket paralysis virus capsid superfamily domain, an RNA helicase domain, a peptidase C3 superfamily domain, and an RNA-dependent RNA polymerase (RdRp) domain. LDIV1 replicates in the host insect and activates small interfering RNA (siRNA)-based host antiviral immunity. Phylogenetic analysis demonstrated that LDIV1 is most closely related to an unspecified member of the order Picornavirales, with 61.7% sequence identity in the RdRp region and 57.6% sequence identity in the coat protein region, and thus meets the demarcation criteria for new species in the genus Iflavirus. To the best of our knowledge, LDIV1 is the first virus discovered in L. delicatula.

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Data availability

Raw reads generated by transcriptomic and sRNA sequencing have been deposited in the Genome Warehouse of the National Genomics Data Center (https://bigd.big.ac.cn/gwh) under accession number PRJCA009021.

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Funding

This research was supported by the National Natural Science Foundation of China (U20A2036) and the Ningbo Science and Technology Innovation 2025 Major Project (2019B10004).

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Authors and Affiliations

  1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro‑products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China

    Yi-Zhe Wang, Zhuang-Xin Ye, Jian‑Ping Chen, Chuan‑Xi Zhang, Jun‑Min Li & Hai-Jian Huang

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  1. Yi-Zhe Wang
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  2. Zhuang-Xin Ye
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  3. Jian‑Ping Chen
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  4. Chuan‑Xi Zhang
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  5. Jun‑Min Li
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  6. Hai-Jian Huang
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Correspondence to Hai-Jian Huang.

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Supplementary Information

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705_2022_5503_MOESM1_ESM.tif

Assembly of RNA-seq reads to obtain the Lycorma delicatula iflavirus 1 (LDIV1) genome sequence. The RNA-seq dataset of L. delicatula (accession number: SRR5134712) was retrieved from NCBI SRA repository and mapped to the LDIV1 genome. (TIF 3480 KB)

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Wang, YZ., Ye, ZX., Chen, J. et al. Complete genome analysis of a novel iflavirus from the spotted lanternfly Lycorma delicatula. Arch Virol 167, 2079–2083 (2022). https://doi.org/10.1007/s00705-022-05503-w

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  • DOI: https://doi.org/10.1007/s00705-022-05503-w

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