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Characterization and complete genome analysis of a novel Escherichia phage, vB_EcoM-RPN242

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Abstract

The novel Escherichia phage vB_EcoM-RPN242 was isolated using a strain of Escherichia coli originating from a diarrheic piglet as a host. The phage was able to form plaques on the E. coli lawn at 15–45 °C. Moreover, it was stable over a wide pH (4–10) and temperature (4–70 °C) range. The vB_EcoM-RPN242 genome was found to be a linear, double-stranded DNA consisting of 154,840 base pairs. There were 195 protein-encoding genes and two tRNAs detected in the genome; however, no genes associated with virulence, toxins or antimicrobial resistance were found. According to overall nucleotide sequence comparisons, vB_EcoM-RPN242 possibly represents a new species in the genus Agtrevirus.

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Acknowledgements

Laurence Crouch is credited with grammar and language proofreading.

Funding

This work was supported by Kasetsart University Research and Development Institute, KURDI grant no. FF(KU)17.64.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Sanamchandra Palace Campus, Nakhon Pathom, 73000, Thailand

    Napakhwan Imklin, Pattaraporn Sriprasong & Rujikan Nasanit

  2. Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhonpathom, Thailand

    Narut Thanantong

  3. Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand

    Porntippa Lekcharoensuk

  4. Center for Advanced Studies in Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, 10900, Thailand

    Porntippa Lekcharoensuk

Authors
  1. Napakhwan Imklin
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  2. Pattaraporn Sriprasong
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  3. Narut Thanantong
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  4. Porntippa Lekcharoensuk
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  5. Rujikan Nasanit
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Contributions

The study conception, design and supervision were contributed by Rujikan Nasanit. Material preparation was performed by Napakhwan Imklin, Pattaraporn Sriprasong, and Narut Thanantong. Data collection and analysis were performed by Napakhwan Imklin and Pattaraporn Sriprasong. Funding acquisition and research program management were contributed by Porntippa Lekcharoensuk. The first draft of the manuscript was written by Napakhwan Imklin. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Porntippa Lekcharoensuk or Rujikan Nasanit.

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The authors have no relevant financial or non-financial interests to disclose.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: T. K. Frey.

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Imklin, N., Sriprasong, P., Thanantong, N. et al. Characterization and complete genome analysis of a novel Escherichia phage, vB_EcoM-RPN242. Arch Virol 167, 1675–1679 (2022). https://doi.org/10.1007/s00705-022-05479-7

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  • DOI: https://doi.org/10.1007/s00705-022-05479-7

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