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Salmonella phage akira, infecting selected Salmonella enterica Enteritidis and Typhimurium strains, represents a new lineage of bacteriophages

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Abstract

Some serovars of Salmonella can cause life-threatening diarrhoeal diseases and bacteriemia. The emergence of multidrug-resistant strains has led to a need for alternative treatments such as phage therapy, which requires available, well-described, diverse, and suitable phages. Phage akira was found to lyse 19 out of 32 Salmonella enterica serovars and farm isolates tested, although plaque formation was observed with only two S. Enteritidis and one S. Typhimurium strain. Phage akira encodes anti-defence genes against type 1 R-M systems, is distinct (<65% nucleotide sequence identity) from related phages and has siphovirus morphology. We propose that akira represents a new genus in the class Caudoviricetes.

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Availability of data and material

The genome sequence of Salmonella phage akira is available in the GenBank database under the accession number NC_054647.1. All data generated or analysed during this study are included in this published article and its supplementary files.

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Acknowledgements

The authors are grateful to Herning Vand (DANVA) for the wastewater sample, the Danish Veterinary and Food Administration for providing the Salmonella strains, and Laura Forero-Junco (KU, DK) for help with SpacePHARER.

Funding

This research was funded by Villum Experiment Grant 17595, Aarhus University Research Foundation AUFF Grant E-2015-FLS-7-28 (Witold Kot), and Human Frontier Science Program Grant RGP0024/2018 (Lars H. Hansen).

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Authors and Affiliations

  1. Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark

    Nikoline S. Olsen, Lars Hestbjerg Hansen & Witold Kot

  2. Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 26, 1958, Frederiksberg, Denmark

    René Lametsch

  3. Institute for Microbiology and Biotechnology, Max Rubner-Institut, Hermann-Weigmann-Str. 1, 24103, Kiel, Denmark

    Natalia Wagner

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Contributions

NSO, LHH, and WK contributed to the study conception and design. Material preparation, data collection, and analysis were performed by NSO, RL, and NW. The first draft of the manuscript was written by NSO, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Nikoline S. Olsen or Witold Kot.

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Olsen, N.S., Lametsch, R., Wagner, N. et al. Salmonella phage akira, infecting selected Salmonella enterica Enteritidis and Typhimurium strains, represents a new lineage of bacteriophages. Arch Virol 167, 2049–2056 (2022). https://doi.org/10.1007/s00705-022-05477-9

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