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Characterization and genome analysis of Pseudomonas aeruginosa phage vB_PaeP_Lx18 and the antibacterial activity of its lysozyme

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Abstract

A lytic Pseudomonas aeruginosa phage, vB_PaeP_Lx18 (Lx18), was isolated from the sewage of a dairy farm. Biological characterization revealed that Lx18 was stable from 40 °C to 60 °C and over a wide range of pH values from 4 to 10. It was able to lyse 63.6% (21/33) of the P. aeruginosa strains tested and was able to reduce and disperse biofilms, with a biofilm reduction rate of 76.8%. Whole-genome sequencing showed that Lx18 is a dsDNA virus with a genome of 42,735 bp and G+C content of 62.16%. The genome contains 54 open reading frames (ORFs), 28 of which have known functions, including DNA replication and modification, transcriptional regulation, structural and packaging proteins, and host cell lysis. No virulence or tRNA genes were identified. Phylogenetic analysis showed that phage Lx18 belongs to the genus Phikmvvirus. The lysozyme of Lx18, Lys18, was cloned and expressed. The combined action of Lys18 and ethylenediaminetetraacetic acid (EDTA) had antibacterial activity against Pseudomonas aeruginosa. The study of phage Lx18 and its lysozyme will provide basic information for further research on the treatment of Pseudomonas aeruginosa infections.

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Acknowledgements

We gratefully acknowledge Dr Zongzhu Liu for collecting samples for the experiments, and we thank the staff of the Central Lab of Qingdao Agricultural University for their assistance with TEM.

Funding

This study was supported by Shandong Province modern agricultural industrial technology system cattle industry innovation team project (No. SDAIT-09-03).

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Authors and Affiliations

  1. College of Veterinary Medicine, Qingdao Agricultural University, Shandong, 266109, China

    Yin Yin, Xinwei Wang, Zehua Mou, Huiying Ren, Can Zhang, Ling Zou, Huanqi Liu, Wenhua Liu & Zongzhu Liu

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  1. Yin Yin
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Contributions

WL conceived and designed the experiments and critically evaluated the manuscript. YY carried out the data analysis and wrote the manuscript. XW and ZM carried out the experiments. HR evaluated the experimental design and helped with experiments and the revision of manuscript. CZ evaluated the experimental design and gave advice about the manuscript. LZ provided reagents and instruments. HL provided financial support for the experiments. All authors read and approved the final manuscript.

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Correspondence to Huanqi Liu or Wenhua Liu.

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Yin, Y., Wang, X., Mou, Z. et al. Characterization and genome analysis of Pseudomonas aeruginosa phage vB_PaeP_Lx18 and the antibacterial activity of its lysozyme. Arch Virol 167, 1805–1817 (2022). https://doi.org/10.1007/s00705-022-05472-0

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