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Full genome sequence of a novel iflavirus from the leafhopper Recilia dorsalis

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Abstract

The leafhopper Recilia dorsalis (family Cicadellidae, tribe Deltocephalini) is a common pest of rice and a transmitter of various viruses. Here, we discovered a novel virus in an R. dorsalis sample and determined its complete genome sequence by metagenomic sequencing and rapid amplification of cDNA ends. Based on a homology search and phylogenetic analysis, we show that the new virus belongs to the genus Iflavirus, family Iflaviridae, and we have tentatively named it “Recilia dorsalis iflavirus 1” (RdIV1). Excluding the polyA tail, the RdIV1 genome is 10,986 nucleotides in length and is predicted to encode a 3,195-amino-acid-long polyprotein that possesses the typical domains of iflaviruses: two rhinovirus-like (rhv-like) capsid domains, a cricket paralysis virus–like (CRPV-like) capsid domain, a helicase domain, a protease domain, and an RNA-dependent RNA polymerase (RdRp) domain. BLAST searches showed that the RdIV1 genome has the highest amino sequence identity (73.8%) in the coat protein region to Euscelidius variegatus virus 1 (EVV-1), a member of to the genus Iflavirus, indicating that RdIV1 can be classified as a new iflavirus.

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Funding

This work was supported by the National Key Research and Development Program of China (2019YFC1200503) and funds from Fujian Agriculture and Forestry University.

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Author notes

  1. These authors contributed equally: Rui Ma and Binghua Nie.

Authors and Affiliations

  1. College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Rui Ma

  2. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Binghua Nie & Jiajia Chen

  3. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Keliang Lv

  4. Center for Agroforestry Mega Data Science, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Jiajing Xiao & Renyi Liu

Authors
  1. Rui Ma
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  2. Binghua Nie
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  3. Jiajia Chen
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  4. Keliang Lv
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  5. Jiajing Xiao
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  6. Renyi Liu
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Contributions

RL designed the research. RM and JX analyzed the data. RM, BN, JC, and KL performed the experiments. RM, JX, and RL wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Renyi Liu.

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The authors have no relevant financial or non-financial interests to disclose.

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This article does not contain any studies with human participants performed by any of the authors.

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Ma, R., Nie, B., Chen, J. et al. Full genome sequence of a novel iflavirus from the leafhopper Recilia dorsalis. Arch Virol 167, 1593–1596 (2022). https://doi.org/10.1007/s00705-022-05464-0

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  • DOI: https://doi.org/10.1007/s00705-022-05464-0

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