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Whole genome sequence of an uncommon G9P[4] species A rotavirus containing DS-1-like (genotype 2) genes in Japan

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Abstract

Species A rotaviruses (RVAs) have been recognized as one of the leading causes of acute gastroenteritis in humans worldwide. Here, the complete coding sequences of 11 RNA segments of an uncommon G9P[4] RVA strain, which was detected in feces of a diarrheal child in Japan, were determined by next-generation sequencing technology. Its genomic constellation, VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5, was determined as G9-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2. This work reports the complete coding sequences of a G9P[4] RVA strain containing DS-1-like (genotype 2) genes that was isolated in Japan in 2013.

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Data availability

The GenBank accession numbers for the complete coding regions of all 11 segments of strain RVA/Human-wt/JPN/11584/2013/G9P[4] are OM301627- OM301637.

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Acknowledgements

We thank Ms. Onda-Shimizu Yuko for her technical help. This study was supported by Grants-in-Aid for Japan Agency for Medical Research and Development (AMED ) under grant numbers JP22fk0108122 and JP22wm0225006.

Funding

This study was supported by Grants-in-Aid for Japan Agency for Medical Research and Development (AMED ) under grant numbers JP22fk0108122 and JP22wm0225006.

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Authors and Affiliations

  1. Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA

    Tung Phan

  2. Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

    Riona Hatazawa & Satoshi Komoto

  3. Nishimura Clinic, Maizuru, Kyoto, Japan

    Shuichi Nishimura

  4. Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

    Pattara Khamrin & Niwat Maneekarn

  5. Division of Microbiology, Nihon University School of Medicine, Itabashi-ku, Tokyo, Japan

    Ngan Thi Kim Pham, Shoko Okitsu, Satoshi Hayakawa & Hiroshi Ushijima

  6. Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan

    Takashi Kobayashi

Authors
  1. Tung Phan
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  2. Riona Hatazawa
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  4. Shuichi Nishimura
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  11. Hiroshi Ushijima
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Contributions

Hiroshi Ushijima, Satoshi Komoto, Takashi Kobayashi, and Niwat Maneekarn designed the study. Riona Hatazawa, Ngan Thi Kim Pham, Pattara Khamrin, and Satoshi Hayakawa performed experiments. Tung Phan, Shoko Okitsu, and Hiroshi Ushijima wrote the manuscript. Shuichi Nishimura provided the specimen. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Hiroshi Ushijima.

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Conflict of interest

The authors have no relevant financial or non-financial interests to disclose.

Research involving human participants and/or animals

The study was approved by the ethics committee of Nihon University (29-9-0, 29-9-1).

Informed consent

Informed consent was obtained from the patient’s parents.

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Handling Editor: Tim Skern.

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Phan, T., Hatazawa, R., Komoto, S. et al. Whole genome sequence of an uncommon G9P[4] species A rotavirus containing DS-1-like (genotype 2) genes in Japan. Arch Virol 167, 1603–1606 (2022). https://doi.org/10.1007/s00705-022-05462-2

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  • DOI: https://doi.org/10.1007/s00705-022-05462-2

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