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Characterization of a new potyvirus infecting Thevetia ahouai in Ecuador

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Abstract

A new potyvirus was found in Thevetia ahouai L. (Fam. Apocynaceae) plants exhibiting white spots on leaves and fruit discoloration in Ecuador. The complete genome sequences of two isolates of this virus, tentatively named "thevetia white spot virus" (ThWSV), were determined and found to be 9,912 (isolate 1) and 9,904 (isolate 2) nucleotides (nt) in length, each encoding a polyprotein of 363 kDa. Sequence comparisons between the two isolates showed 80 and 87% identity at the nt and amino acid (aa) level, respectively, whereas the overall sequence identity between ThWSV and its closest relative was 69% and 71% at the nt and aa level, respectively.

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Fig. 1
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Data availability

The genomic sequences of the two virus isolates reported here have been deposited in the GenBank database under accession numbers OM263475 and OM263476.

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Acknowledgements

The authors acknowledge Dr. Benham Lockhart and the University of Minnesota Imaging Center for assistance with virus purification and particle visualization at the transmission electron microscopy facility.

Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Facultad de Ciencias de la Vida, Escuela Superior Politécnica del Litoral, ESPOL, Km 30.5 Vía Perimetral Campus Gustavo Galindo, Guayaquil, Ecuador

    Maria G. Cañada-Bautista, Edison G. Reyes-Proaño & Diego F. Quito-Avila

  2. Centro de Investigaciones Biotecnológicas del Ecuador, Escuela Superior Politécnica del Litoral, CIBE, Km 30.5 Vía Perimetral Campus Gustavo Galindo, Guayaquil, Ecuador

    Juan F. Cornejo-Franco & Diego F. Quito-Avila

  3. Department of Botany and Plant Pathology, 4575 Research Way Lab, Corvallis, OR, 97333, USA

    Robert A. Alvarez-Quinto

  4. USDA-ARS, Horticultural Crops Disease and Pest Management Research Unit, Corvallis, OR, 97331, USA

    Dimitre Mollov

Authors
  1. Maria G. Cañada-Bautista
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  2. Edison G. Reyes-Proaño
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  3. Juan F. Cornejo-Franco
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  4. Robert A. Alvarez-Quinto
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  5. Dimitre Mollov
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  6. Diego F. Quito-Avila
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Contributions

Study conception and design: DFQ-A. Material preparation, data collection, and analysis were performed by MGC-B, EGR-P, JFC-F, RAA-Q, and DM. The first draft of the manuscript was written by MGC-B and DFQ-A. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Diego F. Quito-Avila.

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Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors. Plant samples were collected under Genetic Resource Access Permit # MAE-DNB-CM-2018-0098, granted by the Department of Biodiversity of the Ecuadorean Ministry of the Environment.

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Handling Editor: Ioannis E. Tzanetakis.

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Supplementary Information

Below is the link to the electronic supplementary material.

705_2022_5450_MOESM1_ESM.jpg

Online Resource 1 Transmission electron micrograph of partially purified extracts from symptomatic leaves of Thevetia ahouai. A representative single flexuous filamentous virus-like particle is shown. The scale bar equals 100 nm. Magnification, 60,000 X. (JPG 4678 KB)

Supplementary file2 (TXT 20 KB)

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Cañada-Bautista, M.G., Reyes-Proaño, E.G., Cornejo-Franco, J.F. et al. Characterization of a new potyvirus infecting Thevetia ahouai in Ecuador. Arch Virol 167, 1461–1466 (2022). https://doi.org/10.1007/s00705-022-05450-6

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  • DOI: https://doi.org/10.1007/s00705-022-05450-6

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