Abstract
In the present study, we analyzed the chemokine-binding protein (CBP) and the GM-CSF/IL-2 inhibition factor (GIF) of orf virus (ORFV) isolates of sheep and goat origin from different geographical regions of India. Both are immunomodulatory proteins known for their unique strategy of establishing short-term immunity and re-infection in their host. The GIF gene is highly conserved, whereas the CBP gene is highly variable. Both the proteins have conserved potential N-glycosylation sites. The GIF protein contains the "WDPWV" motif responsible for receptor activation. In addition, the SUSHI/short consensus repeats (SCR) domain is reported for the first time in ORFV. Both proteins could potentially be used as immunotherapeutic agents in inflammatory diseases related to the overexpression of specific cytokines.
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Acknowledgements
The authors thank the Director, Indian Veterinary Research Institute, for providing necessary facilities and funds to carry out this work, and the staff of Poxvirus Laboratory, IVRI, Mukteswar, for their valuable contributions. The authors also thank the directors and staff of the regional disease diagnostic laboratories of the state animal husbandry departments for sending clinical samples. This study is part of the MVSc thesis work of the first author.
Funding
This study was funded by Department of Biotech nology, Government of India under DBT-NER Twinning project (Grant No. BT/385/NE/TBP/2012).
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Supplementary Fig. S1
Phylogenetic analysis of poxviruses based on CBP and GIF gene sequences obtained from GenBank (Supplementary Table S2). The phylogenetic trees were constructed by the neighbor-joining method in MEGA version 6.0, with 1000 bootstrap replicates.
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Karki, M., Venkatesan, G., Kumar, A. et al. Genetic analysis of two viroceptor genes of orf virus. Arch Virol 167, 1577–1582 (2022). https://doi.org/10.1007/s00705-022-05447-1
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DOI: https://doi.org/10.1007/s00705-022-05447-1