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Characterization of a novel mitovirus isolated from the phytopathogenic fungus Fusarium pseudograminearum

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Abstract

Mycoviruses are widespread in all major groups of plant-pathogenic fungi. So far, only one mycovirus has been reported to be associated with Fusarium pseudograminearum, the causal agent of Fusarium crown rot of wheat. In this study, a double-stranded RNA (dsRNA) segment was isolated from F. pseudograminearum strain JW2-1, and the sequence of its full-length cDNA (3077 nucleotides) was determined. Sequence analysis using the fungal mitochondrial genetic code (UGA coding for tryptophan) indicated that a single large open reading frame (ORF) is present on the positive strand of this dsRNA segment. The ORF encodes a putative RNA-dependent RNA polymerase (RdRp) of 748 amino acids (aa) with a molecular mass of 83.46 kDa. BLASTp analysis revealed that its aa sequence was 28.49–44.03% identical to those of viruses of the family Mitoviridae, with the most similarity to the corresponding RdRp sequences of Ophiostoma mitovirus 1c (44.03% identity) and Ophiostoma mitovirus 1b (40.33% identity). Phylogenetic analysis showed that this mycovirus, designated as "Fusarium pseudograminearum mitovirus 1" (FpgMV1), should be classified as a member of a new species in the earlier proposed genus "Duamitovirus" within the family Mitoviridae. To our best of our knowledge, this is the first report of a mitovirus infecting F. pseudograminearum.

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Acknowledgments

This work was financially supported by the Wheat Industry Technology System of Shandong Province (SDAIT-01-10) and the Agricultural Science and Technology Innovation Project of SAAS (CXGC2021B13 and CXGC2021A33). Thanks to Dr. Xuehong Wu from the College of Plant Protection, China Agricultural University, for reviewing the manuscript prior to submission.

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Authors and Affiliations

  1. Institute of Plant Protection, Shandong Academy of Agricultural Sciences/Shandong Key Laboratory of Plant Virology, Jinan, 250100, People’s Republic of China

    Guoping Ma, Bo Zhang, Kai Qi, Yueli Zhang, Liguo Ma, Hang Jiang & Junshan Qi

  2. College of Life Sciences, Shandong Normal University, Jinan, 250014, People’s Republic of China

    Shujun Qin

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  1. Guoping Ma
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  2. Bo Zhang
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Correspondence to Junshan Qi.

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Ma, G., Zhang, B., Qi, K. et al. Characterization of a novel mitovirus isolated from the phytopathogenic fungus Fusarium pseudograminearum. Arch Virol 167, 1369–1373 (2022). https://doi.org/10.1007/s00705-022-05430-w

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  • DOI: https://doi.org/10.1007/s00705-022-05430-w

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