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Complete genome analysis of the newly isolated Vibrio phage vB_VpP_WS1 of the family Microviridae

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Abstract

Here, we describe the characterization and genome annotation of the newly isolated lytic Vibrio parahaemolyticus phage vB_VpP_WS1, isolated from sewage samples collected in Qingdao, China. Transmission electron microscopy revealed that vB_VpP_WS1 is about 22 nm in size and that the virions are isometric, likely icosahedral, particles similar to those of members of the Microviridae. The digestion patterns of phage nucleic acids and whole-genome sequencing analysis together revealed that phage vB_VpP_WS1 has a single-stranded DNA genome of 5564 nt. Eight open reading frames were identified, only four of which could be annotated. The proteins of vB_VpP_WS1 displayed low sequence similarity to their homologs encoded by other microviruses. Phylogenetic analysis based on the major capsid protein suggested that vB_VpP_WS1 is a tentative new member of the family Microviridae.

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Acknowledgements

This work was supported by the National Science Foundation of China (31870166), the National Key Research and Development (R&D) Program of China (2017YFC1600703), and the China Agriculture Research System (CARS-47).

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Authors and Affiliations

  1. Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China

    Wenjing Xu, Guanhua Xuan, Hong Lin & Jingxue Wang

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  1. Wenjing Xu
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  2. Guanhua Xuan
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  3. Hong Lin
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  4. Jingxue Wang
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Correspondence to Jingxue Wang.

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Xu, W., Xuan, G., Lin, H. et al. Complete genome analysis of the newly isolated Vibrio phage vB_VpP_WS1 of the family Microviridae. Arch Virol 167, 1311–1316 (2022). https://doi.org/10.1007/s00705-022-05413-x

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  • DOI: https://doi.org/10.1007/s00705-022-05413-x

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