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A high-resolution melting assay to differentiate a peste des petits ruminants virus vaccine strain from field isolates in Turkey

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Abstract

Peste des petits ruminants (PPR) mostly affects small ruminants. Vaccination with attenuated vaccines derived from PPR virus (PPRV) provides successful protection against the disease. However, current molecular methods are unable to distinguish vaccine strains from field strains. In this study, we used an EvaGreen-based high-resolution melting (HRM) assay to differentiate a PPRV vaccine strain (Nigeria 75/1) from Turkish field isolates of lineage IV based on melting peaks and melting profiles.

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Acknowledgments

The study was derived from a master's thesis titled ‘‘Molecular Differentiation of Vaccine and Field Strains of Peste des Petits Ruminants Virus’’.

Funding

This study was funded by the Republic of Turkey Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies.

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Authors and Affiliations

  1. Molecular Microbiology Laboratory, Veterinary Control Institute, 42090, Konya, Turkey

    Mustafa Emin Oz

  2. Department of Molecular Biology and Genetics, Faculty of Science, Necmettin Erbakan University, 42090, Konya, Turkey

    Emrah Torlak

Authors
  1. Mustafa Emin Oz
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  2. Emrah Torlak
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Correspondence to Mustafa Emin Oz.

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The authors have no conflicts of interest to declare that are relevant to the content of this article.

Ethical approval

This study using nucleic acid extracts did not involve the handling of live animals.

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Handling Editor: Bert K. Rima.

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Oz, M.E., Torlak, E. A high-resolution melting assay to differentiate a peste des petits ruminants virus vaccine strain from field isolates in Turkey. Arch Virol 167, 941–946 (2022). https://doi.org/10.1007/s00705-022-05386-x

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  • DOI: https://doi.org/10.1007/s00705-022-05386-x

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