Abstract
Dioscorea mosaic associated virus (DMaV) is a member of the genus Sadwavirus, family Secoviridae, that is associated with mosaic symptoms in Dioscorea rotundata in Brazil. The genome of a DMaV isolate detected in D. trifida in Guadeloupe was sequenced by high-throughput sequencing. Using an RT-PCR-based detection assay, we found that DMaV infects D. alata, D. bulbifera, D. cayenensis-rotundata, D. esculenta, and D. trifida accessions conserved in Guadeloupe and Côte d’Ivoire and displays a very high level of molecular diversity in a relatively small region of the genome targeted by the assay. We also provide evidence that DMaV is also present in D. rotundata in Benin and in D. alata in Nigeria.
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Acknowledgements
The authors wish to thank Yacouba Bakayoko and Amani Michel Kouakou for providing samples from the CNRA germplasm collection, Nadia Adjano-Lubin and Eric Bandou for technical help, and David Lange for assistance with sampling.
Funding
This work was funded by the Agence Nationale de la Recherche (ANR) and the Guadeloupe Region through the NetBiome program under project SafePGR (ANR-11-EBIM-0002-06) and by the European Commission and the Guadeloupe Region through the Malin project (2018-FED-1088) under the EU’s Seventh Framework Programme.
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Umber, M., Filloux, D., Svanella-Dumas, L. et al. Host range and molecular variability of the sadwavirus dioscorea mosaic associated virus. Arch Virol 167, 917–922 (2022). https://doi.org/10.1007/s00705-022-05379-w
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DOI: https://doi.org/10.1007/s00705-022-05379-w