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Immunogenicity of an inactivated novel goose parvovirus vaccine for short beak and dwarfism syndrome in Cherry Valley ducks

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Abstract

Duck short beak and dwarfism syndrome (SBDS) is a viral infectious disease caused by novel goose parvovirus (NGPV), which has been responsible for serious economic losses to the Chinese duck industry in recent years. Currently, there is no effective vaccine against this disease. In this study, we developed an inactivated virus vaccine candidate for SBDS based on NGPV strain DS15 isolated from a duck in China. Immune efficacy was evaluated in 112 ducks, which were randomly divided into vaccination, challenge-control, vaccination-challenge, and blank control groups (28 per group). Clinical characteristics, antibodies, virus excretion, viremia, and pathological changes were monitored. No morbidity or death was observed in the immunized ducks, which showed normal weight and a good mental state. High levels of serum antibodies (optical density at 450 nm of ~ 0.63) were detected in ducks immunized with the inactivated vaccine at 7 days post-vaccination (dpv), and the titer of virus-neutralizing antibodies increased from 1:23 to 1:28.5 from 7 to 42 dpv. Measurement of the viral load in anal swab, serum, and tissue samples showed that vaccination significantly inhibited the replication of NGPV in immunized ducks. Moreover, NGPV could not be isolated from the spleens of immunized or vaccinated and challenged ducks. Collectively, these results demonstrate that the newly developed inactivated NGPV vaccine, administered in an oil emulsion adjuvant, possesses good immunogenicity and represents a potentially powerful tool for SBDS prevention and control.

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Funding

This work was supported by the Key Research Project of National Science and Technology (grant numbers 2016YFD0500108, 2016YFD0501003) and the Chinese Natural Sciences Foundation (Grant number 31672572).

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Authors and Affiliations

  1. Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China

    Jiewen Zhou & Xin Guo

  2. Innovation Team of Small Animal Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, 200241, China

    Jiewen Zhou, Chuanfeng Li, Aoxing Tang, Hang Li, Zhaorong Yu, Zongyan Chen & Guangqing Liu

  3. Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai JiaoTong University, 200240, Shanghai, China

    Jiewen Zhou

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  1. Jiewen Zhou
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  2. Chuanfeng Li
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  3. Aoxing Tang
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  4. Hang Li
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  5. Zhaorong Yu
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  6. Zongyan Chen
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  7. Xin Guo
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  8. Guangqing Liu
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Correspondence to Xin Guo or Guangqing Liu.

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The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

All animal experiments were approved by the Animal Welfare and Ethics Committee of Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (approval number SHVRI-ZD-2019-032).

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Zhou, J., Li, C., Tang, A. et al. Immunogenicity of an inactivated novel goose parvovirus vaccine for short beak and dwarfism syndrome in Cherry Valley ducks. Arch Virol 167, 881–889 (2022). https://doi.org/10.1007/s00705-021-05352-z

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  • DOI: https://doi.org/10.1007/s00705-021-05352-z

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