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Complete genome sequences of two isolates of spiraea yellow leafspot virus (genus Badnavirus) from Spiraea x bumalda ‘Anthony Waterer’

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Abstract

The complete genome sequences of two isolates of spiraea yellow leafspot virus (SYLSV) were determined. Spiraea (Spiraea x bumalda) ‘Anthony Waterer’ plants showing virus-like symptoms including yellow spotting and leaf deformation were used for sequencing. The viral genome of SYLSV-MN (Minnesota) and SYLSV-MD (Maryland) is 8,017bp in length. The sequences share 95% identity at the nucleotide level. Both isolates have the same genome organization containing three open reading frames (ORFs), with ORF3 being the largest, encoding a putative polyprotein of 232 kDa with conserved domains including a zinc finger, pepsin-like aspartate protease, reverse transcriptase (RT), and RNase H. Pairwise comparisons between members of the genus Badnavirus showed that gooseberry vein banding associated virus GB1 (HQ852248) and rubus yellow net virus isolate Baumforth's Seedling A (KM078034) were the closest related virus sequences to SYLSV, sharing 73% identity at the nucleotide level. Bacilliform virions with dimensions of 150 nm × 30 nm were observed in virus preparations from symptomatic, but not asymptomatic, plants.

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Funding

This work was supported by the USDA National Institute of Food and Agriculture, Hatch project #1009997 and the University of Minnesota, Twin Cities. Alvarez-Quinto R.A is supported by the Republic of Ecuador through the Ecuadorean Science and Technology Secretariat (SENESCYT).

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Authors and Affiliations

  1. Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN, 55108, USA

    R. A. Alvarez-Quinto, Joana Serrano & B. E. L. Lockhart

  2. Department of Plant Biology, University of Minnesota, St. Paul, MN, 55108, USA

    Neil Olszewski

  3. National Germplasm Resources Laboratory, USDA-ARS, Beltsville, MD, USA

    Samuel Grinstead

  4. USDA-ARS Horticultural Crops Research Unit, Corvallis, OR, 97331, USA

    Dimitre Mollov

Authors
  1. R. A. Alvarez-Quinto
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  2. Joana Serrano
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  3. Neil Olszewski
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  4. Samuel Grinstead
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  5. Dimitre Mollov
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  6. B. E. L. Lockhart
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Corresponding author

Correspondence to R. A. Alvarez-Quinto.

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Alvarez-Quinto, R.A., Serrano, J., Olszewski, N. et al. Complete genome sequences of two isolates of spiraea yellow leafspot virus (genus Badnavirus) from Spiraea x bumalda ‘Anthony Waterer’. Arch Virol 167, 631–634 (2022). https://doi.org/10.1007/s00705-021-05336-z

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  • DOI: https://doi.org/10.1007/s00705-021-05336-z

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