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Identification and genome analysis of a tomato zonate spot virus isolate from Bidens pilosa

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Abstract

Bidens pilosa is a weed species that invades crop areas in tropical and subtropical regions. To date, only two potyviruses have been reported to infect B. pilosa. Here, we report the complete genome sequence of a tomato zonate spot tospovirus (TZSV) isolate from Bidens named TZSV-Bidens. The tripartite RNA of the TZSV-Bidens genome contains L, M, and S segments that are 8912, 4724, and 2997 nt in length, respectively. The genome contains five open reading frames (ORFs), with 92.23–95.01% amino acid sequence identity to the TZSV-YN isolate. Phylogenetic analysis based on amino acid sequences of members of the family Tospoviridae showed that TZSV-Bidens was grouped into a well-supported Eurasian cluster. The intergenic regions (IGRs) of the M and S RNAs are among the most variable regions and are far shorter than those of the TZSV-YN reference genome.

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Data presented in this manuscript are freely available upon request from the corresponding author.

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Acknowledgements

This work was supported by Grants from the Guizhou Province Technology R&D Program ([2019]2398), the Guizhou University Education Foundation ([2020]67), and the National Natural Science Foundation of China (Grant No. 31760503).

Funding

This work was supported by Grants from the Guizhou Province Technology R&D Program ([2019]2398), Guizhou University Education Foundation ([2020]67), and the National Natural Science Foundation of China (Grant No. 31760503).

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Authors and Affiliations

  1. Institute of Crop Protection, College of Agriculture, Guizhou University, Guiyang, 550025, Guizhou, China

    Tengzhi Xu, Xiangru Chen, Rongyu Li, Xiaomao Wu & Youhua Long

  2. Guizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang, 550008, Guizhou, China

    Lei Lei

  3. Guizhou Academy of Tobacco Sciences, Guiyang, 550001, Guizhou, China

    Meng-ao Jia

Authors
  1. Tengzhi Xu
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  2. Lei Lei
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  3. Xiangru Chen
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  4. Rongyu Li
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  5. Xiaomao Wu
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  6. Youhua Long
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  7. Meng-ao Jia
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Contributions

TX, LL, and MJ designed the study. TX, LL, XC, RL, XW, YL, and MJ performed the experiments. TX, LL, and MJ analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Meng-ao Jia.

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The authors declare that they have no competing interests.

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Xu, T., Lei, L., Chen, X. et al. Identification and genome analysis of a tomato zonate spot virus isolate from Bidens pilosa. Arch Virol 167, 625–630 (2022). https://doi.org/10.1007/s00705-021-05330-5

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  • DOI: https://doi.org/10.1007/s00705-021-05330-5

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