Abstract
Paper mulberry vein banding virus (PMVBV), a member of the genus Badnavirus in the family Caulimoviridae, infects paper mulberry (Broussonetia papyrifera), a dicotyledonous plant. Putative promoter regions in the PMVBV genome were tested using recombinant plant expression vectors, revealing that the promoter activity of three genome fragments was about 1.5-fold higher than that of the 35S promoter of cauliflower mosaic virus in Nicotiana benthamiana. In transformed transgenic Arabidopsis thaliana plants, these promoter constructs showed constitutive expression. Based on the activity and gene expression patterns of these three promoter constructs, a fragment of 384 bp (named PmVP) was deduced to contain the full-length promoter of the PMVBV genome. The results suggest that the PMVBV-derived promoter can be used for the constitutive expression of transgenes in dicotyledonous plants.
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05 December 2021
“Typographical error corrected. Redundant '??' before and after 'β-glucuronidase' deleted.”.
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This work was supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (no. 18KJA210001) and the Doctor Startup Fund Program of Jiangsu University of Science and Technology (no. 1732931601).
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The nucleotide sequence reported in this article has been submitted to the GenBank database under accession number MW052244.
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Smith, W.K., Ma, Y., Yu, J. et al. Characterization of a strong constitutive promoter from paper mulberry vein banding virus. Arch Virol 167, 163–170 (2022). https://doi.org/10.1007/s00705-021-05310-9
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DOI: https://doi.org/10.1007/s00705-021-05310-9