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Complete genome sequence of a new orthotospovirus associated with ringspot in Fatsia japonica

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Abstract

Fatsia japonica is an evergreen shrub native to Japan. For decades, virus-like ringspot symptoms have been observed on leaves of F. japonica in Japan; however, previous attempts to identify the causal agents have been unsuccessful. In this study, we detected an orthotospovirus-like sequence in symptomatic F. japonica plants using RNA sequencing analysis. The complete nucleotide sequences of the L, M, and S segments of the virus were determined using conventional sequencing strategies. The virus had a typical orthotospovirus genome structure, and the putative nucleocapsid protein showed the highest sequence identity to that of groundnut chlorotic fan-spot virus, with 83.7% identity at the amino acid level (which is below the 90% species demarcation cutoff for the genus Orthotospovirus). Although we could not confirm the pathogenicity of the virus in F. japonica due to difficulties associated with mechanical inoculation, its association with the observed symptoms was suggested by the fact that the virus was detected only in symptomatic leaf areas. Based on these results, we consider this virus, which we have named "Fatsia japonica ringspot-associated virus" (FjRSaV), to be the first representative of a new orthotospovirus species, for which we propose the binomial "Orthotospovirus fatsiae".

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Data availability

The complete genome sequence of FjRSaV-YA1 has been deposited in the DNA Data Bank of Japan under the accession numbers LC626335, LC626336, and LC626337 for the L, M, and S segment, respectively.

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Authors and Affiliations

  1. Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Yugo Kitazawa, Takamichi Nijo, Masanobu Nishikawa, Oki Matsumoto, Takumi Suzuki, Masato Suzuki, Kensaku Maejima, Shigetou Namba & Yasuyuki Yamaji

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  1. Yugo Kitazawa
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  2. Takamichi Nijo
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  3. Masanobu Nishikawa
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  4. Oki Matsumoto
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  5. Takumi Suzuki
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  6. Masato Suzuki
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  7. Kensaku Maejima
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  8. Shigetou Namba
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  9. Yasuyuki Yamaji
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Correspondence to Yasuyuki Yamaji.

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Kitazawa, Y., Nijo, T., Nishikawa, M. et al. Complete genome sequence of a new orthotospovirus associated with ringspot in Fatsia japonica. Arch Virol 167, 615–618 (2022). https://doi.org/10.1007/s00705-021-05300-x

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