Abstract
A novel mycovirus was identified infecting the phytopathogenic fungus Microdochium albescens. The characterized dsRNA segment, corresponding to the replicative intermediate of the mitovirus genome, is 2,562 base pairs (bp) in length, with an A+U content of 62.3%. A single open reading frame (ORF) was identified, encoding a putative RNA-dependent RNA polymerase (RdRp) of 706 amino acids. Phylogenetic analysis showed that this virus should be classified as a member of a new species in the genus Mitovirus, family Mitoviridae, for which we propose the name "Mitovirus gaucho". This is the first report of a mycovirus infecting the phytopathogenic fungus M. albescens, the causative agent of leaf scald on rice in Brazil.
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We acknowledge the members of our laboratory for helpful discussions.
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This research was financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brazil (CAPES)—Finance Code 001, CNPq and FAPEMIG. LLL was the recipient of a CNPq undergraduate fellowship (PIBIC/2019-2020/CNPq), and JMMB, RRR, and RSC were recipients of CAPES fellowships.
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Supplementary file2Supplementary Fig. S1 SDT analysis using MUSCLE to align sequences of several mitoviruses from GenBank Refseq (TIF 1348 kb)
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de Rezende, R.R., de Oliveira Souza, F., Leal, L.L. et al. Characterization of a new mitovirus infecting the phytopathogenic fungus Microdochium albescens. Arch Virol 166, 3455–3459 (2021). https://doi.org/10.1007/s00705-021-05242-4
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DOI: https://doi.org/10.1007/s00705-021-05242-4