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Phenotypic and genotypic characterization of the new Bacillus cereus phage SWEP1

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Abstract

A new Bacillus cereus phage, SWEP1, was isolated from black soil. The host lysis activity of phage SWEP1 has a relatively short latent time (20 min) and a small burst size of 83 PFU. The genome of SWEP1 consists of 162,461 bp with 37.77% G+C content. The phage encodes 278 predicted proteins, 103 of which were assigned functionally. No tRNA genes were found. Comparative genomics analysis indicated that SWEP1 is related to Bacillus phage B4 (86.91% identity, 90% query coverage). Phenotypic and genotypic characterization suggested that SWEP1 is a new member of a new species in the genus Bequatrovirus, family Herelleviridae.

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Acknowledgements

We thank David R. Johnson, Josep Ramoneda, and Elyse Stachler for improving the quality and clarity of this manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (41877412), Project to Attract High Level Foreign Experts (G20190001094), the National Key R&D Program of China (2016YFD0200306), the 2115 Talent Development Program of China Agricultural University and China Scholarship Council.

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Author notes

  1. Chujin Ruan and Xinyao Niu contributed equally to this work.

Authors and Affiliations

  1. College of Land Science and Technology, China Agricultural University, Beijing, 100193, China

    Chujin Ruan, Xinyao Niu, Hanqing Wu, Zechao Ma, Kun Zhu & Gang Wang

  2. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China

    Guangzhou Xiong

  3. School of Civil and Hydraulic Engineering, Hefei University of Technology, Hefei, 230009, China

    Guowei Chen

  4. School of Earth and Space Sciences, Peking University, Beijing, 100871, China

    Ying Liu

  5. Department of Environmental Microbiology, Swiss Federal Institute of Aquatic Science and Technology (Eawag), 8600, Dübendorf, Switzerland

    Chujin Ruan

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  1. Chujin Ruan
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Contributions

CJR, GWC, and GW organized and designed experiments; CJR, XYN, HQW, and ZCM performed experiments; CJR, XYN, GZX analyzed data; and CJR, XYN, GZX, and YL wrote the first draft of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Gang Wang.

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Ruan, C., Niu, X., Xiong, G. et al. Phenotypic and genotypic characterization of the new Bacillus cereus phage SWEP1. Arch Virol 166, 3183–3188 (2021). https://doi.org/10.1007/s00705-021-05222-8

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