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Isolation and phylogenetic analysis of strains of feline calicivirus in Beijing, China

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Abstract

Feline calicivirus (FCV) is a contagious cat pathogen that causes oral ulceration and/or upper respiratory disease. In this study, we collected 61 samples from a pet hospital in Beijing and used PCR or RT-PCR to detect FCV and feline herpesvirus 1 (FHV-1). Approximately 44.3% (27/61) of the samples were FCV positive, and 23.0% (14/61) were coinfected with FCV and FHV-1. FCV was isolated from 15 samples. One isolate was from a cat with virulent systemic disease (VSD) signs, and 14 isolates were from cats with stomatitis or upper respiratory diseases. The range of genome sequence identity among these isolates was 76.1–100.0%. Four of the isolates were considered to be of the same strain, with sequence identity ranging from 99.5 to 99.7%, and two isolates, BJ-280 and BJ-288, had completely identical sequences. The genomic sequence identity ranged from 76.0 to 88.5% between the 15 isolates and several reference strains, including the F4 and F9 vaccine strains. These results demonstrate that many FCV strains are co-circulating in Beijing. Due to the diversity of FCV in Beijing, it is necessary to monitor the current prevalence of the virus. This study provides more information for the development of effective measures to control FCV.

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References

  1. Afonso MM, Pinchbeck GL, Smith SL, Daly JM, Gaskell RM, Dawson S, Radford AD (2017) A multi-national European cross-sectional study of feline calicivirus epidemiology, diversity and vaccine cross-reactivity. Vaccine 35:2753–2760

    Article  CAS  Google Scholar 

  2. Alvarez-Sanchez C, Cancio-Lonches C, Mora-Heredia JE, Santos-Valencia JC, Barrera-Vázquez OS, Yocupicio-Monroy M, Gutiérrez-Escolano AL (2015) Negative effect of heat shock on feline calicivirus release from infected cells is associated with the control of apoptosis. Virus Res 198:44–52

    Article  CAS  Google Scholar 

  3. Battilani M, Vaccari F, Carelle MS, Morandi F, Benazzi C, Kipar A, Dondi F, Scagliarini A (2013) Virulent feline calicivirus disease in a shelter in Italy: a case description. Res Vet Sci 95(1):283–290

    Article  Google Scholar 

  4. Brunet S, Sigoillot-Claude C, Pialot D, Poulet H (2019) Multiple correspondence analysis on amino acid properties within the variable region of the capsid protein shows differences between classical and virulent systemic feline calicivirus strains. Viruses 11(12):1090

    Article  CAS  Google Scholar 

  5. Coyne KP, Christley RM, Pybus OG, Dawson S, Gaskell RM, Radford AD (2012) Large-scale spatial and temporal genetic diversity of feline calicivirus. J Virol 86:11356–11367

    Article  CAS  Google Scholar 

  6. Dawson S, Willoughby K, Gaskell RM, Wood G, Chalmers WSK (2001) A field trial to assess the effect of vaccination against feline herpesvirus, feline calicivirus and feline panleucopenia virus in 6-week-old kittens. J Feline Med Surg 3:17–22

    Article  CAS  Google Scholar 

  7. Di Martino B, Lanave G, Di Profio F, Melegari I, Marsilio F, Camero M, Catella C, Capozza P, Bányai K, Barrs VR, Buonavoglia C, Martella V (2020) Identification of feline calicivirus in cats with enteritis. Transbound Emerg Dis 67:2579–2588

    Article  Google Scholar 

  8. Guo H, Miao Q, Zhu J, Yang Z, Liu G (2018) Isolation and molecular characterization of a virulent systemic feline calicivirus isolated in China. Infect Genet Evol 65:425–429

    Article  CAS  Google Scholar 

  9. Henzel A, SeS M, Luo S, Lovato LT, Weiblen R (2012) Genetic and phylogenetic analyses of capsid protein gene in feline calicivirus isolates from Rio Grande do Sul in southern Brazil. Virus Res 163:667–671

    Article  CAS  Google Scholar 

  10. Hou J, Sánchez-Vizcaíno F, McGahie D, Lesbros C, Almeras T, Howarth D, O’Hara V, Dawson S, Radford AD (2016) European molecular epidemiology and strain diversity of feline calicivirus. Vet Rec 178:114–115

    Article  CAS  Google Scholar 

  11. Hurley KE, Pesavento PA, Pedersen NC, Poland AM, Wilson E, Foley JE (2004) An outbreak of virulent systemic feline calicivirus disease. J Am Vet Med Assoc 224:241–249

    Article  Google Scholar 

  12. Liu C, Liu Y, Qian P, Cao Y, Wang J, Sun C, Huang B, Cui N, Huo N, Wu H, Wang L, Xi X, Tian K (2020) Molecular and serological investigation of cat viral infectious diseases in China from 2016 to 2019. Transbound Emerg Dis 67:2329–2335

    Article  CAS  Google Scholar 

  13. Lu Z, Ledgerwood ED, Hinchman MM, Dick R, Parker JSL (2018) Conserved surface residues on the feline calicivirus capsid are essential for interaction with its receptor feline junctional adhesion molecule A (fJAM-A). J Virol 92(8):e00035-e118

    Article  Google Scholar 

  14. Makino A, Shimojima M, Miyazawa T, Kato K, Tohya Y, Akashi H (2006) Junctional adhesion molecule 1 is a functional receptor for feline calicivirus. J Virol 80:4482–4490

    Article  CAS  Google Scholar 

  15. Ossiboff RJ, Parker JS (2007) Identification of regions and residues in feline junctional adhesion molecule required for feline calicivirus binding and infection. J Virol 81:13608–13621

    Article  CAS  Google Scholar 

  16. Pedersen NC, Elliott JB, Glasgow A, Poland A, Keel K (2000) An isolated epizootic of hemorrhagic-like fever in cats caused by a novel and highly virulent strain of feline calicivirus. Vet Microbiol 73:281–300

    Article  CAS  Google Scholar 

  17. Pesavento PA, MacLachlan NJ, Dillard-Telm L, Grant CK, Hurley KF (2004) Pathologic, immunohistochemical, and electron microscopic findings in naturally occurring virulent systemic feline calicivirus infection in cats. Vet Pathol 41:257–263

    Article  CAS  Google Scholar 

  18. Pesavento PA, Stokol T, Liu H, van der List DA, Gaffney PM, Parker JS (2011) Distribution of the feline calicivirus receptor junctional adhesion molecule a in feline tissues. Vet Pathol 48:361–368

    Article  CAS  Google Scholar 

  19. Radford AD, Dawson S, Ryvar R, Coyne K, Johnson DR, Cox MB, Acke EF, Addie DD, Gaskell RM (2003) High genetic diversity of the immunodominant region of the feline calicivirus capsid gene in endemically infected cat colonies. Virus Genes 27:145–155

    Article  CAS  Google Scholar 

  20. Seal BS, Neill JD (1995) Capsid protein gene sequence of feline calicivirus isolates 255 and LLK: further evidence for capsid protein configuration among feline caliciviruses. Virus Genes 9:183–187

    Article  CAS  Google Scholar 

  21. Smith SL, Afonso MM, Pinchbeck GL, Gaskell RM, Dawson S, Radford AD (2020) Temporally separated feline calicivirus isolates do not cluster phylogenetically and are similarly neutralised by high-titre vaccine strain FCV-F9 antisera in vitro. J Feline Med Surg 22:602–607

    Article  Google Scholar 

  22. Spiri AM, Thézé J, Meli ML, Cattori V, Berger A, Steinrigl A, Pybus OG, Hofmann-Lehmann R, Willi B (2016) Genetic diversity and phenotypic associations of feline caliciviruses from cats in Switzerland. J Gen Virol 97:3253–3266

    Article  CAS  Google Scholar 

  23. Stuart AD, Brown TDK (2007) Alpha 2,6-linked sialic acid acts as a receptor for Feline calicivirus. J Gen Virol 88:177–186

    Article  CAS  Google Scholar 

  24. Sun Y, Deng M, Peng Z, Hu R, Chen H, Wu B (2017) Genetic and phylogenetic analysis of feline calicivirus isolates in China. Vet J (London, England: 1997) 220:24–27

    Article  CAS  Google Scholar 

  25. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  Google Scholar 

  26. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  Google Scholar 

  27. Tian J, Liu D, Liu Y, Wu H, Jiang Y, Zu S, Liu C, Sun X, Liu J, Qu L (2016) Molecular characterization of a feline calicivirus isolated from tiger and its pathogenesis in cats. Vet Microbiol 192:110–117

    Article  CAS  Google Scholar 

  28. Wang K, Pei Z, Hu G (2017) First report of Feline Calicivirus (FCV) infection in stray cats in northeast China. Pol J Vet Sci 20:595–598

    Article  CAS  Google Scholar 

  29. Zhao Y, Chen X, Ying Y, Wang K, Dong H, Gao C, Yang S, Hu G (2017) Isolation and phylogenetic analysis of three feline calicivirus strains from domestic cats in Jilin Province, China. Adv Virol 162:2579–2589

    CAS  Google Scholar 

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Funding

This study was funded by the National Key Research and Development Program of China (2016YFD0501003).

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Author notes

  1. Zhaoyang Wang and Ting Xin contributed equally to this work.

Authors and Affiliations

  1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China

    Zhaoyang Wang, Ting Xin, Yajun Jiang, Xueting Liu, Xiaoyu Guo, Weifeng Yuan, Hongfei Zhu & Hong Jia

  2. China Institute of Veterinary Drug and Control, NO. 8, Zhongguancun South Street, Haidian District, Beijing, 100081, China

    Jin Wei

  3. Guanzhong Animal Hospital Chain Agencies, Ganlu Xi Yuan, Chaoyang District, Beijing, 100025, China

    Wenjing Song

  4. Animal Disease Prevention and Control Center of Fengtai District, Beijing, 100071, China

    Yanbin Cui

Authors
  1. Zhaoyang Wang
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  2. Ting Xin
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  3. Jin Wei
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  4. Yajun Jiang
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  5. Xueting Liu
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  8. Weifeng Yuan
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  9. Yanbin Cui
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  10. Hongfei Zhu
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  11. Hong Jia
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Corresponding author

Correspondence to Hong Jia.

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The authors declare that they have no conflicts of interest.

Ethical approval

Animal experiments (sampling) were approved by the Animal Care and Use Committee of the Institute of Animal Sciences of the Chinese Academy of Agricultural Sciences. Sampling and data release were approved by the animals’ owners.

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Handling Editor: Reimar Johne.

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Wang, Z., Xin, T., Wei, J. et al. Isolation and phylogenetic analysis of strains of feline calicivirus in Beijing, China. Arch Virol 166, 2521–2527 (2021). https://doi.org/10.1007/s00705-021-05163-2

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  • DOI: https://doi.org/10.1007/s00705-021-05163-2

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