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A novel temperate phage, vB_PstS-pAN, induced from the naphthalene-degrading bacterium Pseudomonas stutzeri AN10

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Abstract

A novel temperate phage named vB_PstS-pAN was induced by mitomycin C treatment from the naphthalene-degrading bacterium Pseudomonas stutzeri AN10. The phage particles have icosahedral heads and long non-contractile tails, and vB_PstS-pAN can therefore be morphologically classified as a member of the family Siphoviridae. The whole genome of vB_PstS-pAN is 39,466 bp in length, with an 11-nt 3’ overhang cohesive end. There are 53 genes in the vB_PstS-pAN genome, including genes responsible for phage integration, replication, morphogenesis, and bacterial lysis. The vB_PstS-pAN genome has low similarity to other phage genomes in the GenBank database, suggesting that vB_PstS-pAN is a novel member of the family Siphoviridae.

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Availability of data and material

The complete genome sequence of vB_PstS-pAN is available in the GenBank database under the accession number MW651859.

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Acknowledgements

This work was supported by the National Key R&D Program of China (2018YFA0902100 and 2018YFA0902103), and the National Natural Science Foundation of China (91951204).

Funding

This work was supported by the National Key R&D Program of China (2018YFA0902100 and 2018YFA0902103), and the National Natural Science Foundation of China (91951204).

Author information

Authors and Affiliations

  1. College of Engineering, Peking University, Beijing, 100871, China

    Zhou Feng, Xinwu Liu, Miaoxiao Wang, Yong Nie & Xiao-Lei Wu

  2. Institute of Ocean Research, Peking University, Beijing, 100871, China

    Xiao-Lei Wu

  3. Institute of Ecology, Peking University, Beijing, 100871, China

    Xiao-Lei Wu

  4. PKU and CUPB MEOR Research Center, Peking University, Beijing, 100871, China

    Xiao-Lei Wu

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  1. Zhou Feng
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  3. Miaoxiao Wang
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Contributions

X-LW and YN designed the study. ZF, XL, and MW performed experiments and analyzed data. The first draft of the manuscript was written by ZF. All authors read and approved the final manuscript.

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Correspondence to Yong Nie or Xiao-Lei Wu.

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Feng, Z., Liu, X., Wang, M. et al. A novel temperate phage, vB_PstS-pAN, induced from the naphthalene-degrading bacterium Pseudomonas stutzeri AN10. Arch Virol 166, 2267–2272 (2021). https://doi.org/10.1007/s00705-021-05098-8

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