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Phylogenetic analysis of classical swine fever virus isolates from China

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Abstract

Classical swine fever (CSF), caused by classical swine fever virus (CSFV), is a severe disease that causes huge economic losses in the swine industry worldwide. In China, CSF has been under control due to extensive vaccination since 1954. However, there are still sporadic CSF outbreaks in China. Here, we isolated 27 CSFV strains from three Chinese provinces (Shaanxi, Gansu, and Ningxia) from 2011 to 2018. Phylogenetic analysis based on the full-length envelope glycoprotein E2 coding region revealed that 25 out of 27 CSFV isolates clustered within subgroups 2.1 and 2.2, while two strains from Gansu belonged to subgroup 1.1. The sequence identity among these 27 isolates varied from 79.3% to 99.8% (nucleotides) and from 83.1% to 99.7% (amino acids). Further analysis based on the E2 amino acid sequences showed that these new isolates have consistent amino acid substitutions, including R31K and N34S.

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Data availability

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This research was supported by funds from the Programs for Science and Technology of Xianyang City [2019k02-63].

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Author notes

  1. Xiaofu Zhu and Mingjie Liu contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Animal Epidemic Disease Diagnostic Laboratory of Molecular Biology in Xianyang City, Xianyang Vocational Technical College, Xianyang, 712000, Shaanxi, China

    Xiaofu Zhu & Xujin Wu

  2. College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Mingjie Liu & Wentao Ma

  3. Yangling Bodeyue Biotechnology Co., Ltd., Yangling, 712100, Shaanxi, China

    Xuanduo Zhao

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  1. Xiaofu Zhu
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  2. Mingjie Liu
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  4. Wentao Ma
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Contributions

XFZ, MJL, and WTM designed the study. XFZ, MJL, XJW, XDZ, and WTM performed the experiments and collected data. MJL analyzed the data. MJL AND XFZ wrote and revised the manuscript. XFZ provided the funding. All authors have read and approved the manuscript.

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Correspondence to Xiaofu Zhu.

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The authors declare that there is no conflict of interest.

Ethical approval

The collection of clinical samples was approved by the Animal Ethics Committee of Xianyang Vocational Technical College and performed in accordance with animal ethics guidelines and approved protocols.

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Handling Editor: Patricia Aguilar.

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Cite this article

Zhu, X., Liu, M., Wu, X. et al. Phylogenetic analysis of classical swine fever virus isolates from China. Arch Virol 166, 2255–2261 (2021). https://doi.org/10.1007/s00705-021-05084-0

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