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Complete genome sequence of a grapevine Roditis leaf discoloration-associated virus (GRLDaV) variant from South Africa

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Abstract

High-throughput sequencing (HTS) was used to construct the virome profile of an old grapevine-leafroll-diseased grapevine (Vitis vinifera). De novo assembly of HTS data showed a complex infection, including a virus sequence with similarity to viruses of the genus Badnavirus, family Caulimoviridae. The complete genome sequence of this virus consists of 7090 nucleotides and has four open reading frames (ORFs). Genome organisation and phylogenetic analysis identify this virus as a divergent variant of grapevine Roditis leaf discoloration-associated virus (GRLDaV) with 90% nucleotide sequence identity to isolate w4 (NC_027131). This is the first genome sequence of a South African variant of GRLDaV.

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Acknowledgements

This study was partially funded by VirFree. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant agreement no. 734736. This publication reflects only the authors’ view. The Agency is not responsible for any use that may be made of the information it contains. The financial assistance of Winetech (P04000069) towards this research is hereby acknowledged.

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Authors and Affiliations

  1. Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa

    Rachelle Bester, Gerhard Pietersen & Hans J. Maree

  2. Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloníki, Greece

    Leonidas Lotos & Varvara I. Maliogka

  3. ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute of the Agricultural Research Council), Stellenbosch, 7599, South Africa

    Abraham Vermeulen

  4. Citrus Research International, Stellenbosch, 7600, South Africa

    Hans J. Maree

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  1. Rachelle Bester
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  2. Leonidas Lotos
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  3. Abraham Vermeulen
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  4. Gerhard Pietersen
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  5. Varvara I. Maliogka
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  6. Hans J. Maree
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Correspondence to Hans J. Maree.

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Bester, R., Lotos, L., Vermeulen, A. et al. Complete genome sequence of a grapevine Roditis leaf discoloration-associated virus (GRLDaV) variant from South Africa. Arch Virol 166, 2041–2044 (2021). https://doi.org/10.1007/s00705-021-05079-x

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  • DOI: https://doi.org/10.1007/s00705-021-05079-x

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