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Molecular characterization of a novel single-stranded RNA virus, ChRV1, isolated from the plant-pathogenic fungus Colletotrichum higginsianum

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Abstract

In this study, a novel single-stranded RNA virus was isolated from the plant-pathogenic fungus Colletotrichum higginsianum strain HTC-5, and the virus was named “Colletotrichum higginsianum ssRNA virus 1” (ChRV1). The complete genome of ChRV1 is 3850 nucleotides in length with a GC content of 52% and contains two in-frame open reading frames (ORFs): ORF1 (smaller) and ORF2 (larger). ORF1 encodes a protein with the highest sequence similarity to proteins encoded by Phoma matteucciicola RNA virus 1 (PmRV1, 47.99% identity) and Periconia macrospinosa ambiguivirus 1 (PmAV1, 50.73% identity). ORF2 encodes a protein with a conserved RNA-dependent RNA polymerase (RdRp) domain with similarity to the RdRps of PmRV1 (61.41% identity) and PmAV1 (60.61% identity), which are recently reported unclassified (+) ssRNA mycoviruses. Phylogenetic analysis of the RdRp domain showed that ChRV1 grouped together with PmRV1, PmAV1, and other unclassified (+) ssRNA mycoviruses and had a distant relationship to invertebrate viruses and plant viruses of the family Tombusviridae. This is the first report of a novel (+) ssRNA virus infecting the phytopathogenic fungus C. higginsianum.

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Funding

This research was supported by the Excellent Doctoral Dissertation Cultivating Fund of Hunan Agricultural University (YB2019005) and the Scientific Research Fund of Hunan Provincial Education Department (19K040).

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Authors and Affiliations

  1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha, 410128, Hunan, People’s Republic of China

    Hong Liu, Hui Wang & Qian Zhou

  2. Agricultural Science Institute of Xiang Xi Tujia and Miao Autonomous Prefecture, Xiangxi, 416000, China

    Xun Lu, Cheng Xiao & Bo Peng

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  1. Hong Liu
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  2. Hui Wang
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  3. Xun Lu
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  4. Cheng Xiao
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  5. Bo Peng
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  6. Qian Zhou
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Correspondence to Qian Zhou.

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Handling Editor: Ioly Kotta-Loizou.

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Liu, H., Wang, H., Lu, X. et al. Molecular characterization of a novel single-stranded RNA virus, ChRV1, isolated from the plant-pathogenic fungus Colletotrichum higginsianum. Arch Virol 166, 1805–1809 (2021). https://doi.org/10.1007/s00705-021-05071-5

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  • DOI: https://doi.org/10.1007/s00705-021-05071-5

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