Abstract
We identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions.
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References
Mei Y, Veras S, Dietzgen R, Kitajima EW (2016) A nucleorhabdovirus associated with yellow leaf blotches in Solanum aculeatissimum in the Amazon basin. Summa Phytopathol 42(suppl.):abst. 128
Kitajima EW (2020) An annotated list of plant viruses and viroids described in Brazil (1926–2018). Biota Neotrop 20:e20190932
Dantas L, Melo A, Pereira P, Souza L, Vasconselos Filho S, Silva F (2017) Histochemical screening of leaves compared to in situ and in vitro calluses of Solanum aculeatissimum Jacq. J Agric Sci 9:80–96
Kuhn JH, Adkins S, Alioto D, Alkhovsky SV, Amarasinghe GK et al (2020) 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornaviriae), including the large orders Bunyavirales and Mononegavirales. Arch Virol. https://doi.org/10.1007/s00705-020-04731-2
Dietzgen RG, Kondo H, Goodin MM, Kurath G, Vasilakis N (2017) The family Rhabdoviridae: mono- and bipartite negative-sense RNA viruses with diverse genome organization and common evolutionary origins. Virus Res 227:158–170
Walker PJ, Dietzgen RG, Joubert DA, Blasdell KR (2011) Rhabdovirus accessory genes. Virus Res 162:110–125
Wang Q, Ma X, Qian S, Zhou X, Sun K, Chen X et al (2015) Rescue of a plant negative-strand RNA virus from cloned cDNA: Insights into enveloped plant virus movement and morphogenesis. PLoS Pathog 11:e1005223
Jackson AO, Dietzgen RG, Goodin MM, Brag JN, Deng M (2005) Biology of plant rhabdoviruses. Annu Rev Phytopathol 43:623–660
Hawkes JG, Lester RN, Nee M, Estrada N (1991) Solanaceae II: taxonomy chemistry and evolution. The Royal Botanic Gardens, Kew, p 483
Lamprecht RL, Pietersen G, Kasdorf GGF, Nel LH (2009) Characterisation of a proposed nucleorhabdovirus new to South Africa. Eur J Plant Pathol 123:105–123
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549
Kosugi S, Hasebe M, Matsumura N, Takashima H, Miyamoto-Sato E, Tomita M, Yanagawa H (2009) Six classes of nuclear localization signals specific to different binding grooves of importin α. J Biol Chem 284:478–485
Whitfield AE, Huot OB, Martin KM, Kondo H, Dietzgen RG (2018) Plant rhabdoviruses—their origins and vector interactions. Curr Opin Virol 33:198–207
Dietzgen RG, Bejerman NE, Goodin MM, Higgins CM, Huot OB, Kondo H, Martin KM, Whitfield AE (2020) Diversity and epidemiology of plant rhabdoviruses. Virus Res 281:197942
Bejerman N, Debat H, Dietzgen RG (2020) The plant negative-sense RNA virosphere: virus discovery through new eyes. Front Microbiol 11:588427
Bandyopadhyay A, Kopperud K, Anderson G, Martin K, Goodin M (2010) An integrated protein localization and interaction map for Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus. Virology 402:61–71
Jang C, Wang R, Wells J, Leon F, Farman M, Hammond J, Goodin MM (2017) Genome sequence variation in the constricta strain dramatically alters the protein interaction and localization map of Potato yellow dwarf virus. J Gen Virol 98:1526–1536
Pappi PG, Dovas CI, Efthimiou KE, Maliogka VI, Katis NI (2013) A novel strategy for the determination of a rhabdovirus genome and its application to sequencing of Eggplant mottled dwarf virus. Virus Genes 47:105–113
Babaie G, Habibi MK, Massah A, Dizadji A, Izadinejad L, Simon A (2015) Complete genome sequence and genome analysis of Eggplant mottled dwarf virus-Iranian isolate. J Phytopathol 163:331–341
Gaafar YZA, Abdelgalil MAM, Knierim D, Richert-Pöggler KR, Menzel W, Winter S, Ziebell H (2018) First report of physostegia chlorotic mottle virus on tomato (Solanum lycopersicum) in Germany. Plant Dis 102:255
Menzel W, Richert-Pöggler KR, Winter S, Knierim D (2018) Characterization of a nucleorhabdovirus from Physostegia. Acta Hortic 1193:29–37
Anderson G, Wang R, Bandyopadhyay A, Goodin M (2012) The nucleocapsid protein of potato yellow dwarf virus: protein interactions and nuclear import mediated by a non-canonical nuclear localization signal. Front Plant Sci 3:14
Anderson G, Jang C, Wang R, Goodin M (2018) Mapping the nuclear localization signal in the matrix protein of potato yellow dwarf virus. J Gen Virol 99:743–752
Funding
This work was jointly supported by the Queensland Government Department of Agriculture and Fisheries and the University of Queensland. Additional support was provided by FAPESP Grants: 2017/50222-0, Sprint Fapesp/UQ (2017/50039) and Biota Program (2017/18910-4).
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The authors have no conflict of interest or competing interests.
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Appropriate permits for import of biological materials were obtained from the Australian Government Department of Agriculture, Water and the Environment. Cloning and sequencing of the JYBaV genome was done under permit from the University of Queensland Institutional Biosafety Committee. This article does not contain any studies with human participants or animals performed by any of the authors.
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The annotated genome sequence generated and analysed during the current study is available in the DDBJ/EMBL/GenBank databases under the accession number MW014292.
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Dietzgen, R.G., Bejerman, N.E., Mei, Y. et al. Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil. Arch Virol 166, 1615–1622 (2021). https://doi.org/10.1007/s00705-021-05040-y
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DOI: https://doi.org/10.1007/s00705-021-05040-y