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Low virus diversity and spread in wild Capsicum spp. accessions from Ecuador under natural inoculum pressure

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Abstract

Challenging wild plant accessions with pathogens is an initial approach for finding resistance genes for breeding programs. Viruses can be transmitted artificially by mechanical or arthropod-borne inoculation, but these experimental assays do not always reproduce natural conditions in the field. In this study, 56 wild Capsicum spp. accessions from Ecuador that were under natural inoculum pressure for six months were screened for virus infections by RNA sequencing. These plants exhibited low virus diversity in comparison to a commercial pepper cultivar that was used as a susceptible host. Subjecting numerous plants to natural infection prior to artificial assays may indicate promising accessions to track within virus/vector resistance breeding programs.

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Availability of data and material

The datasets generated during and/or analysed during the current study are available in the NCBI repository, https://www.ncbi.nlm.nih.gov/nucleotide

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Acknowledgements

We thank the “Acuerdo de transferencia de material para el uso de semillas de Capsicum spp. del banco de germoplasma para fines de investigación entre el Instituto Nacional de Investigaciones (INIAP) y la Universidad Técnica de Manabí (UTM) de fecha septiembre 16 de 2016”.

Funding

This work was supported by Secretaría de Educación Superior, Ciencia, Tecnología e Innovación del Ecuador (SENESCYT), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Apoio à Pesquisa do Distrito Federal (FAP-DF).

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Authors and Affiliations

  1. Departamento de Ciencias Agronómicas, Facultad de Ingeniería Agronómica, Universidad Técnica de Manabí, Portoviejo, 130105, Ecuador

    Jefferson B. Vélez-Olmedo & Liliana Corozo Quiñonez

  2. Departamento de Fitopatologia, Universidade de Brasília, Brasília, DF, Brasil

    Jefferson B. Vélez-Olmedo & Sergio M. Vélez-Zambrano

  3. Carrera de Ingeniería Agrícola, Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López, Campus Politécnico El Limón, Km 2.7 Vía, Calceta-El, Limón, Ecuador

    Sergio M. Vélez-Zambrano

  4. Departamento de Recursos Fitogenéticos (DENAREF) Estación Experimental Santa Catalina, Instituto Nacional de Investigaciones Agropecuarias (INIAP), Quito, Ecuador

    Álvaro Monteros-Altamirano

  5. Departamento de Biologia Celular, Universidade de Brasília, Brasília, Brasil

    Athos S. De Oliveira & Renato O. Resende

Authors
  1. Jefferson B. Vélez-Olmedo
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  5. Athos S. De Oliveira
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  6. Renato O. Resende
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by JBV-O, LC, SMV-Z, ÁM-A, and ASO. The first draft of the manuscript was written by JBV-O, ASO, and ROR, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Athos S. De Oliveira.

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Vélez-Olmedo, J.B., Quiñonez, L.C., Vélez-Zambrano, S.M. et al. Low virus diversity and spread in wild Capsicum spp. accessions from Ecuador under natural inoculum pressure. Arch Virol 166, 1447–1453 (2021). https://doi.org/10.1007/s00705-021-05027-9

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  • DOI: https://doi.org/10.1007/s00705-021-05027-9

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