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Nuclear localization of non-structural protein 3 (NS3) during dengue virus infection

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Abstract

Although dengue virus (DENV) replication occurs in the cytoplasm, the nucleus plays an essential role during infection. Both the capsid protein (C) and non-structural protein 5 (NS5) are translocated into the infected cell nucleus to favor viral replication. Previously, our group reported the nuclear localization of the NS3 protein during DENV infection of mosquito cells; however, the nuclear localization of the DENV NS3 protein in human host cells has not been described. Here, we demonstrated that NS3 is present in the nucleus of Huh7 cells at early infection times, and later, it is mainly located in the cytoplasm.

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Acknowledgements

The authors thank Dr. Bibiana Chávez-Munguía and Anel E. Lagunes Guillen for their assistance in preparation of the electron microscopy image samples, Fernando Medina and Cleotilde Cancio Lonches for their assistance in cell culture, and Jaime Zarco for technical assistance. This research was supported by CONACYT (Mexico) grants CB-220824 and A1-S-9005 from RMDA and CB-250696 from ALGE, and Fundación Miguel Alemán. The funders had no role in study design, data collection, analysis, decision to publish, or manuscript preparation. Palacios-Rápalo SN, De Jesús-González LA, Reyes-Ruiz JM, Farfan-Morales CN, and Osuna-Ramos JF received scholarships from CONACYT.

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  1. Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico

    Selvin Noé Palacios-Rápalo, Luis Adrián De Jesús-González, José Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Ana Lorena Gutiérrez-Escolano & Rosa María del Ángel

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  1. Selvin Noé Palacios-Rápalo
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Palacios-Rápalo, S.N., De Jesús-González, L.A., Reyes-Ruiz, J.M. et al. Nuclear localization of non-structural protein 3 (NS3) during dengue virus infection. Arch Virol 166, 1439–1446 (2021). https://doi.org/10.1007/s00705-021-05026-w

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