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Biology and genetic diversity of phasey bean mild yellows virus, a common virus in legumes in Australia

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Abstract

This study examined the natural and experimental host range and aphid and graft transmission of the tentative polerovirus phasey bean mild yellows virus (PBMYV). Eleven complete coding sequences from PBMYV isolates were determined from a range of hosts and locations. We found two genetically distinct variants of PBMYV. PBMYV-1 was the originally described variant, and PBMYV-2 had a large putative recombination in open reading frame 5 such that PBMYV-1 and PBMYV-2 shared only 65-66% amino acid sequence identity in the P5 protein. The virus was transmitted by a clonal colony of cowpea aphids (Aphis craccivora) and by grafting with infected scions but was not transmitted by a clonal colony of green peach aphids (Myzus persicae). PBMYV was found in natural infections in 11 host species with a range of symptoms and severity, including seven important grain legume crops from across a wide geographic area in Australia. PBMYV was common and widespread in the tropical weed phasey bean (Macroptilium lathyroides), but it is likely that there are other major alternative hosts for the virus in temperate regions of Australia. The experimental host range of PBMYV included the Fabaceae hosts chickpea (Cicer arietinum), faba bean (Vicia faba), pea (Pisum sativum), and phasey bean, but transmissions failed to infect several other members of the families Asteraceae, Cucurbitaceae, Fabaceae and Solanaceae. PBMYV was commonly found in grain legume crops in eastern and western Australia, sometimes at greater than 90% incidence. This new knowledge about PBMYV warrants further assessments of its economic impact on important grain legume crops.

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Genome sequence data have been deposited in the GenBank database. Reference virus isolates are lodged in the Plant Virus Collection, Department of Agriculture and Fisheries, Queensland.

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Acknowledgements

We gratefully acknowledge the contributions of Dr. Alison Dann, Mr. Peter Cross, Mr. Shane Hossel, and Dr. Tamilarasan Thangavel. Dr. Joop van Leur, Dr. John Thomas, and Ms. Judy Parry kindly provided virus-infected samples for this study.

Funding

This work was supported by the Department of Agriculture and Fisheries, Queensland, the Tasmanian Institute of Agriculture, and funds from the Australian Grains Research and Development Corporation projects DAQ00186 and DAN00202. Dr. Appiah was recipient of an Australian Development Scholarship provided by AusAID.

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Authors and Affiliations

  1. Department of Agriculture, Fisheries and Forestry, Ecosciences Precinct, GPO Box 267, Brisbane, QLD, 4001, Australia

    Murray Sharman & Fiona Filardo

  2. Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, Legon, Accra, Ghana

    Andrew S. Appiah

  3. Department of Jobs, Precincts and Regions, Agriculture Victoria, Grains Innovation Park, 110 Natimuk Road, Horsham, VIC, 3400, Australia

    Narelle Nancarrow & Mohammad Aftab

  4. Department of Primary Industries and Regional Development, Industry and Economic Development, 3 Baron-Hay Court, Kensington, WA, 6151, Australia

    Benjamin S. Congdon

  5. Department of Primary Industries and Regional Development, DPIRD Diagnostic Laboratory Services, South Perth, WA, Australia

    Monica Kehoe

  6. Tasmanian Institute of Agriculture, University of Tasmania, New Town, Hobart, TAS, Australia

    Robert S. Tegg & Calum R. Wilson

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  1. Murray Sharman
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Correspondence to Murray Sharman.

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Sharman, M., Appiah, A.S., Filardo, F. et al. Biology and genetic diversity of phasey bean mild yellows virus, a common virus in legumes in Australia. Arch Virol 166, 1575–1589 (2021). https://doi.org/10.1007/s00705-021-05022-0

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