Abstract
Potato aucuba mosaic virus (PAMV), a positive single-strand RNA virus, has one of the longest genomes of the viruses in the genus Potexvirus. In 2019, potato samples with mottle and crinkling symptoms from Huzhou, Zhejiang province, China, were identified to be infected with PAMV, potato virus X (PVX), and potato virus Y (PVY) by transcriptome sequencing. To study the effects of single infection by PAMV, the full-length sequence of PAMV from Huzhou (MT193476) was determined and an infectious full-length cDNA clone was constructed. This cDNA clone was infectious by agro-infiltration, leading to systemic symptoms in Nicotiana benthamiana, tomato, pepper, and potato.
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Acknowledgements
This work was financially supported by the National Key Research and Development Program of China (2018YFD0200802), the State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products (KF20190107), the Natural Science Foundation of Zhejiang Province (LY20C140001), the Natural Science Foundation of Ningbo City (2019A610409), the Chinese Agriculture Research System (CARS-24-C-04), and the K.C. Wong Magna Fund of Ningbo University.
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Chen, B., Lin, Q., Yin, Y. et al. Construction of an infectious full-length cDNA clone of potato aucuba mosaic virus. Arch Virol 166, 1427–1431 (2021). https://doi.org/10.1007/s00705-021-05018-w
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DOI: https://doi.org/10.1007/s00705-021-05018-w