Little cherry virus 2 (LChV-2) is a causal agent of little cherry disease, which produces small, misshapen fruit with poor color and taste. As LChV-2 symptoms are only present near harvest, molecular detection is essential for effective control. Therefore, we determined the titer and distribution of this virus in infected trees over time. While initial infections were found to be basipetal, in field trees, early-stage infection was characterized by uneven distribution and low titer, concentrated in woody stems. In contrast, established infections were systemic, and detection was consistent across tissues. These data provide improved sampling recommendations for the detection of LChV-2.
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This project was supported by a grant from the Washington Tree Fruit Research Commission to S.J. Harper. S.T. DuPont and R. Naranjo from WSU Extension and G. Bishop from G.S. Long assisted in sample collection, and S. Szostek assisted in qPCR optimization. The authors also thank D. Villamor and S. Pillai for contributing to the original design of these experiments.
This research was funded by the Washington Tree Fruit Research Commission.
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Wright, A.A., Shires, M.K. & Harper, S.J. Titer and distribution of little cherry virus 2 in Prunus avium. Arch Virol 166, 1415–1419 (2021). https://doi.org/10.1007/s00705-021-05015-z