The complete genome sequence of passiflora virus Y (PaVY) from passion fruit growing in Guangdong province, China, was determined. The entire positive single-strand RNA genome comprises 9681 nucleotides (nt) excluding the poly(A) tail and encodes a polyprotein of 3084 amino acids flanked by 5’ and 3’ untranslated regions of 169 and 257 nt, respectively. In sequence comparisons and phylogenetic analysis, PaVY appears to represent a new species in the bean common mosaic virus subgroup of the genus Potyvirus. This is the first report of the complete genome sequence of PaVY and the first report of this virus in China.
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The nucleotide sequences reported in this manuscript have been deposited in the GenBank database under accession number MW165064 and are available in the Electronic Supplementary Material.
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We thank Prof. M. J. Adams, Minehead, UK, for professional comments and correcting the language of the manuscript. This work was financially supported by the National Key Research and Development Program of China (2019YFD1001801) and K. C. Wong Magna Fund in Ningbo University.
This work was financially supported by the National Key Research and Development Program of China (2019YFD1001801) and K. C. Wong Magna Fund in Ningbo University.
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Supplementary file2 Fig. S1 Disease symptoms and molecular detection of PaVY in passion fruit. (A) PaYY-infected passion fruit leaves with chlorosis, mosaic, and yellow spots from sample 4 from Shaoguan and a healthy leaf (Health) as a control. (B) PCR products of PaVY CP gene separated by 1.5% agarose gel electrophoresis. M, 2K Plus DNA Marker (TransGen Biotech, Beijing, China), lane N, water control; lanes 1-4, -1, -2, -3, and -4 from Shaoguan, Guangdong province, China (TIF 2814 KB)
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Chen, B., Wu, D., Zheng, H. et al. Complete genome sequence of passiflora virus Y infecting passion fruit in China. Arch Virol 166, 1489–1493 (2021). https://doi.org/10.1007/s00705-021-05013-1