Characterisation of an Australian fowlpox virus carrying a near-full-length provirus of reticuloendotheliosis virus

Abstract

Fowlpox virus (FWPV), which is the type member of the genus Avipoxvirus, subfamily Chordopoxvirinae, family Poxviridae, can lead to significant losses to the poultry industry. Although a large number of fowlpox virus genomes have been sequenced and characterised globally, there are no sequences available at the genomic level from Australian isolates. Here, we present the first complete genome sequence of a fowlpox virus vaccine strain (FWPV-S) containing an integrated near-full-length reticuloendotheliosis virus (REV) provirus. The genome of FWPV-S showed the highest sequence similarity to a fowlpox virus from the USA (97.74% identity). The FWPV-S genome contained 16 predicted unique genes, while a further two genes were fragmented compared to previously reported FWPV genome sequences. Subsequent phylogenetic analysis showed that FWPV-S was most closely related to other fowlpox viruses. This is the first reported genome sequence of FWPV from Australia.

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Data availability

The complete sequence of the FWPV-S genome was deposited in the GenBank database under the accession number MW142017.

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Acknowledgements

Dr. Sarker is the recipient of an Australian Research Council Discovery Early Career Researcher Award (Grant number DE200100367) funded by the Australian government. We also gratefully acknowledge the funding contributed by the Australian Biosecurity CRC for Emerging Infectious Disease in support of this work.

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Conceptualization, SS, TRB, DBB; formal analysis, SS, AA, DBB; investigation, SS, TRB, DBB; methodology, SS, TRB, DBB; writing—original draft, SS; writing—review and editing, SS, AA, TRB, DBB. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Subir Sarker.

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Sarker, S., Athukorala, A., Bowden, T.R. et al. Characterisation of an Australian fowlpox virus carrying a near-full-length provirus of reticuloendotheliosis virus. Arch Virol 166, 1485–1488 (2021). https://doi.org/10.1007/s00705-021-05009-x

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