Complete genome sequence of artemisia virus B, a new polerovirus infecting Artemisia princeps in South Korea


The complete genome sequence of a new polerovirus found naturally infecting Artemisia princeps, artemisia virus B (ArtVB), was determined using high-throughput sequencing. The ArtVB genome comprises 6,141 nucleotides and contains six putative open reading frames (ORF0 to ORF5) with a genome structure typical of poleroviruses. A multiple sequence alignment showed that the complete ArtVB genome shares 50.98% nucleotide sequence identity with ixeridium yellow mottle virus 1 (IxYMaV-1, GenBank accession no. KT868949). ArtVB shares the highest amino acid sequence identity in P0 and P3–P5 (21.54%–51.69%) with other known poleroviruses. Phylogenetic analysis indicated that ArtVB should be considered a member of a new species within the genus Polerovirus, family Luteoviridae.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2


  1. 1.

    Domier LL (2012) Family Luteoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, ninth report of the international committee on taxonomy of viruses. Academic Press, San Diego, pp 1045–1053

    Google Scholar 

  2. 2.

    Pagán I, Holmes EC (2010) Long-term evolution of the Luteoviridae: time scale and mode of virus speciation. J Virol 84:6177–6187.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  3. 3.

    Smirnova E, Firth AE, Miller WA, Scheidecker D, Brault V, Reinbold C, Rakotondrafara AM, Chung BYW, Ziegler-Graff V (2015) Discovery of a small non-AUG-initiated ORF in poleroviruses and luteoviruses that is required for long-distance movement. PLoS Pathog 11:e1004868

    Article  Google Scholar 

  4. 4.

    Zhou B, Wang F, Zhang X, Zhang L, Lin H (2017) Sequencing and phylogenetic analysis of tobacco virus 2, a polerovirus from Nicotiana tabacum. Arch Virol. 162:2159–2162

    CAS  Article  Google Scholar 

  5. 5.

    Hirano A, Goto M, Mitsui T, Hashimoto-Hachiya A, Tsuji G, Furue M (2017) Antioxidant Artemisia princeps extract enhances the expression of filaggrin and loricrin via the AHR/OVOL1 pathway. Int J Mol Sci 18:1948

    Article  Google Scholar 

  6. 6.

    Ramel ME, Gugerli P, Besse S, Simonnet X, Balmelli C (2013) Molecular characterization of artemisia virus A, a new sobemovirus isolated from Artemisia annua. Adv Virol 158:463–466

    CAS  Google Scholar 

  7. 7.

    Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549

    CAS  Article  Google Scholar 

  8. 8.

    Bortolamiol D, Pazhouhandeh M, Marrocco K, Genschik P, Ziegler-Graff V (2007) The polerovirus F box protein P0 targets ARGONAUTE1 to suppress RNA silencing. Curr Biol 17:1615–1621

    CAS  Article  Google Scholar 

  9. 9.

    Huang LF, Naylor M, Pallett DW et al (2005) The complete genome sequence, organization and affinities of carrot red leaf virus. Arch Virol 150:1845–1855

    CAS  Article  Google Scholar 

  10. 10.

    King AM, Adams MJ, Carstens EB, Lefkowitz E (2011) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Elsevier, New York

    Google Scholar 

  11. 11.

    Mutterer JD, Stussi-Garaud C, Michler P et al (1999) Role of the beet western yellows virus readthrough protein in virus movement in Nicotiana clevelandii. J Gen Virol 80(Pt 10):2771–2778.

    CAS  Article  PubMed  Google Scholar 

Download references


This research was supported by grants from the National Institute of Forest Science, Republic of Korea (project no. FG0700-2018-02-2198), Rural Development Administration, Republic of Korea (project no. PJ0137412018) and the KRIBB Research Initiative Program, funded by the Ministry of Science and ICT, Republic of Korea (project no. KGM5372012). We are thankful to the Office of Research and Innovation at the Mongolian National University of Education. Also, we thank Steven M. Thompson from Edanz Group ( for editing a draft of this manuscript.

Author information



Corresponding authors

Correspondence to Suk‑Yoon Kwon or Jae Sun Moon.

Ethics declarations

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article did not involve any studies with human participants or animals performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Handling Editor: Robert H.A. Coutts.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 24 KB)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Igori, D., Lim, S., Cho, H.S. et al. Complete genome sequence of artemisia virus B, a new polerovirus infecting Artemisia princeps in South Korea. Arch Virol 166, 1495–1499 (2021).

Download citation