Common bean plants (Phaseolus vulgaris L.) showing different virus-like symptoms were collected in northwestern Argentina. Dot-blot hybridization tests showed that the begomoviruses bean golden mosaic virus and tomato yellow vein streak virus were the most prevalent, but they also revealed the presence of unknown begomoviruses. The complete genome sequence of one of these unknown begomoviruses was determined. Sequence analysis showed that the virus is a typical New World begomovirus, for which the name "bean bushy stunt virus" (BBSV) is proposed. Biological assays based on biolistic inoculations showed that BBSV induced leaf roll and stunting symptoms similar to those observed in the field-collected common bean sample.
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This work was supported by the Argentine Government, through the Instituto Nacional de Tecnología Agropecuaria (INTA) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Pablo Gastón Reyna has a CONICET doctoral scholarship. The authors thank researchers Daniel Agustín Maidana, Juan Facundo Alamo, and Ceferino René Flores for field sampling.
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Reyna, P.G., Bejerman, N., Laguna, I.G. et al. Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina. Arch Virol 166, 1409–1414 (2021). https://doi.org/10.1007/s00705-021-05002-4