Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina

Abstract

Common bean plants (Phaseolus vulgaris L.) showing different virus-like symptoms were collected in northwestern Argentina. Dot-blot hybridization tests showed that the begomoviruses bean golden mosaic virus and tomato yellow vein streak virus were the most prevalent, but they also revealed the presence of unknown begomoviruses. The complete genome sequence of one of these unknown begomoviruses was determined. Sequence analysis showed that the virus is a typical New World begomovirus, for which the name "bean bushy stunt virus" (BBSV) is proposed. Biological assays based on biolistic inoculations showed that BBSV induced leaf roll and stunting symptoms similar to those observed in the field-collected common bean sample.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2

References

  1. 1.

    Aidawati N, Hidayat SH, Hidayat P, Suseno R, Sujiprihati S (2007) Response of various tomato genotypes to begomovirus infection and its improved diagnostic. HAYATI J Biosci 14:93–97. https://doi.org/10.4308/hjb.14.3.93

    Article  Google Scholar 

  2. 2.

    Albuquerque LC, Martin DP, Avila AC, Inoue-Nagata AK (2010) Characterization of tomato yellow vein streak virus, a begomovirus from Brazil. Virus Genes 40:140–147. https://doi.org/10.1007/s11262-009-0426-2

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Alemandri V et al (2015) Three members of the Bemisia tabaci (Hemiptera: Aleyrodidae) cryptic species complex occur sympatrically in Argentine horticultural crops. J Econ Entomol 108:405–413. https://doi.org/10.1093/jee/tov017

    CAS  Article  PubMed  Google Scholar 

  4. 4.

    Barreto SS, Hallwass M, Aquino OM, Inoue-Nagata AK (2013) A study of weeds as potential inoculum sources for a tomato-infecting begomovirus in central Brazil. Phytopathology. 103:436–444. https://doi.org/10.1094/PHYTO-07-12-0174-R

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Blawid R, Fontenele RS, Lacorte C, Ribeiro SG (2013) Molecular and biological characterization of corchorus mottle virus, a new begomovirus from Brazil. Arch Virol 158:2603–2609. https://doi.org/10.1007/s00705-013-1764-x

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Brown JK et al (2015) Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol 160:1593–1619. https://doi.org/10.1007/s00705-015-2398-y

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Fernandes FR et al (2011) Molecular and biological characterization of a new Brazilian begomovirus, euphorbia yellow mosaic virus (EuYMV), infecting Euphorbia heterophylla plants. Arch Virol 156:2063–2069. https://doi.org/10.1007/s00705-011-1070-4

    CAS  Article  PubMed  Google Scholar 

  8. 8.

    Ferro CG et al (2017) The ever increasing diversity of begomoviruses infecting non-cultivated hosts: new species from Sida spp. and Leonurus sibiricus, plus two New World alphasatellites. Ann Appl Biol 170:204–218. https://doi.org/10.1111/aab.12329

    CAS  Article  Google Scholar 

  9. 9.

    Fiallo-Olivé E, Chirinos DT, Castro R, Navas-Castillo J (2018) A novel strain of Pepper leafroll virus infecting common bean and soybean in Ecuador. Plant Dis 164:62–72. https://doi.org/10.1094/PDIS-06-18-1076-PDN

    Article  Google Scholar 

  10. 10.

    Fiallo-Olivé E, Zerbini FM, Navas-Castillo J (2015) Complete nucleotide sequences of two new begomoviruses infecting the wild malvaceous plant Melochia sp. in Brazil. Arch Virol 160:3161–3164. https://doi.org/10.1007/s00705-015-2619-4

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Jullien N (2013) AmplifiX 1.7.0. Aix-Marseille Univ, CNRS, INP, Inst Neurophysiopathol, Marseille

  12. 12.

    Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  13. 13.

    Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evol 1:1–5. https://doi.org/10.1093/ve/vev003

    Article  Google Scholar 

  14. 14.

    Martínez-Ayala A, Sánchez-Campos S, Cáceres F, Aragõn-Caballero L, Navas-Castillo J, Moriones E (2014) Characterisation and genetic diversity of pepper leafroll virus, a new bipartite begomovirus infecting pepper, bean and tomato in Peru. Ann Appl Biol 164:62–72. https://doi.org/10.1111/aab.12074

    CAS  Article  Google Scholar 

  15. 15.

    Morales FJ (2006) History and current distribution of Begomoviruses in Latin America. Adv Virus Res 67:127–162. https://doi.org/10.1016/S0065-3527(06)67004-8

    Article  PubMed  Google Scholar 

  16. 16.

    Morales FJ, Anderson PK (2001) The emergence and dissemination of whitefly-transmitted geminiviruses in Latin America: Brief review. Arch Virol 146:415–441. https://doi.org/10.1007/s007050170153

    CAS  Article  PubMed  Google Scholar 

  17. 17.

    Muhire BM, Varsani A, Martin DP (2014) SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PLoS ONE 9:1–8

    Article  Google Scholar 

  18. 18.

    Passos LS et al (2017) Complete genome sequence of a new bipartite begomovirus infecting Macroptilium lathyroides in Brazil. Arch Virol 162:3551–3554. https://doi.org/10.1007/s00705-017-3522-y

    CAS  Article  PubMed  Google Scholar 

  19. 19.

    Rodríguez-Pardina PE, Arneodo JD, Truol GA, Herrera PS, Laguna IG (2004) First record of Cowpea mild mottle virus in bean crops in Argentina. Aust Plant Pathol. 33:129–130. https://doi.org/10.1071/AP03076

    Article  Google Scholar 

  20. 20.

    Rodríguez-Pardina PE, Hanada K, Laguna IG, Zerbini FM, Ducasse DA (2011) Molecular characterisation and relative incidence of bean- and soybean-infecting begomoviruses in northwestern Argentina. Ann Appl Biol 158:69–78. https://doi.org/10.1111/j.1744-7348.2010.00441.x

    Article  Google Scholar 

  21. 21.

    Rodríguez-Pardina PE, Reyna P, Campos RE, Varela G, Peña-Malavera A, Gerónimo LM (2018) Evaluation of the behavior of common bean cultivars and promising lines under natural virus infection. Hortic Argent 37:13–23 oai:localhost:20.500.12123/4429

  22. 22.

    Rodríguez-Pardina PE, Zerbini F, M and Ducasse D. A, (2006) Genetic diversity of begomoviruses infecting soybean, bean and associated weeds in Northwestern Argentina. Fitopatol Bras 31:342–348. https://doi.org/10.1590/S0100-41582006000400003

    Article  Google Scholar 

  23. 23.

    Rojas MR, Hagen C, Lucas WJ, Gilbertson RL (2005) Exploiting Chinks in the plant’s armor: evolution and emergence of geminiviruses. Annu Rev Phytopathol 43:361–394. https://doi.org/10.1146/annurev.phyto.43.040204.135939

    CAS  Article  PubMed  Google Scholar 

  24. 24.

    Sambrook J, Russell DW (2001) Molecular cloning. Sambrook & Russel, New York

  25. 25.

    Saxena S, Tiwari AK (2017) Begomoviruses: occurrence and management in Asia and Africa. Springer, Singapore. https://doi.org/10.1007/978-981-10-5984-1

    Google Scholar 

  26. 26.

    Sharma PN, Sharma V, Sharma A, Rajput K, Sharma SK (2015) Identification and molecular characterization of Bean yellow mosaic virus infecting French bean in Himachal Pradesh. Virus Dis 26:315–318. https://doi.org/10.1007/s13337-015-0270-z

    CAS  Article  Google Scholar 

  27. 27.

    Singh SP, Schwartz HF (2010) Breeding common bean for resistance to diseases: a review. Crop Sci 50:2199–2223. https://doi.org/10.2135/cropsci2009.03.0163

    Article  Google Scholar 

  28. 28.

    Sverdlov E, Azhikina T (2005) Primer walking. Encycl Life Sci. https://doi.org/10.1038/npg.els.0005382

    Article  Google Scholar 

  29. 29.

    Tavares SS, Ramos-Sobrinho R, González-Aguilera J, Lima GSA, Assunção IP, Zerbini FM (2012) Caracterização Molecular Adicional de Begomovírus Associados a Plantas Daninhas no Brasil, com Ênfase em Sida spp. Planta Daninha 30:305–315. https://doi.org/10.1590/S0100-83582012000200009

    Article  Google Scholar 

  30. 30.

    Vaghi Medina CG, Martin DP, López Lambertini PM (2015) Tomato mottle wrinkle virus, a recombinant begomovirus infecting tomato in Argentina. Arch Virol 160:581–585. https://doi.org/10.1007/s00705-014-2216-y

    CAS  Article  PubMed  Google Scholar 

  31. 31.

    Varela G, Ávalos V, Reyna P, Laguna IG, Rodriguez-Pardina PE (2018) Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update. Austr Plant Pathol. 47:343–350. https://doi.org/10.1007/s13313-018-0563-y

    CAS  Article  Google Scholar 

  32. 32.

    Zambrano K, Fernández-Rodríguez T, Marys E (2012) Molecular characterization of a new begomovirus that infects Euphorbia heterophylla and Solanum lycopersicum in Venezuela. Arch Virol 157:379–382. https://doi.org/10.1007/s00705-011-1157-y

    CAS  Article  PubMed  Google Scholar 

  33. 33.

    Zerbini FM et al (2017) ICTV virus taxonomy profile: Geminiviridae. J Gen Virol 98:131–133. https://doi.org/10.1099/jgv.0.000738

    CAS  Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

This work was supported by the Argentine Government, through the Instituto Nacional de Tecnología Agropecuaria (INTA) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Pablo Gastón Reyna has a CONICET doctoral scholarship. The authors thank researchers Daniel Agustín Maidana, Juan Facundo Alamo, and Ceferino René Flores for field sampling.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Pablo Gastón Reyna.

Ethics declarations

Conflict of interest

There is no conflict of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Handling Editor: Elvira Fiallo-Olivé.

Supplementary Information

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Reyna, P.G., Bejerman, N., Laguna, I.G. et al. Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina. Arch Virol 166, 1409–1414 (2021). https://doi.org/10.1007/s00705-021-05002-4

Download citation