Molecular characterization of a novel partitivirus isolated from the phytopathogenic fungus Aplosporella javeedii

Abstract

Aplosporella javeedii is a pathogenic fungus that causes canker and dieback of jujube in China. In this study, we report a new mycovirus, Aplosporella javeedii partitivirus 1 (AjPV1), isolated from A. javeedii strain NX55-3. The AjPV1 genome contains two double-stranded RNA elements (dsRNA1 and dsRNA2). The size of dsRNA1 is 2,360 bp, and it encodes a putative RNA-dependent RNA polymerase (RdRp), while dsRNA2 is 2,301 bp in length and encodes a putative capsid protein (CP). The sequences of RdRp and CP have significant similarity to those of members of the family Partitiviridae. Sequence alignment and phylogenetic analysis showed that AjPV1 is a new member of the family Partitiviridae that is related to members of the genus Betapartitivirus. To our knowledge, AjPV1 is the first mycovirus reported from A. javeedii.

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Acknowledgements

We express our deep gratitude to Dr. Yanping Fu (Huazhong Agricultural University) and Jiatao Xie (Huazhong Agricultural University) for providing guidance and improving the manuscript.

Funding

This work was financially supported by the National Natural Science Foundation of China (31901934) and the Special Fund for Agro-Scientific Research in the Public Interest (201503110).

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YiZ designed the research. XW, YuZ, YS, CW, HD, HX JC and LY performed the experimental work. YiZ, XW, RZ and CW analyzed the data and wrote the manuscript.

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Correspondence to Caiyi Wen.

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Wan, X., Zhao, Y., Zhang, Y. et al. Molecular characterization of a novel partitivirus isolated from the phytopathogenic fungus Aplosporella javeedii. Arch Virol 166, 1237–1240 (2021). https://doi.org/10.1007/s00705-021-04988-1

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