A novel virus, Botryosphaeria dothidea bipartite mycovirus 1 (BdBMV1), was isolated from the plant-pathogenic fungus Botryosphaeria dothidea strain HNDT1, and the complete nucleotide sequence of its genome was determined. BdBMV1 consists of two genomic segments. The first segment is 1,976 bp in length and contains a single open reading frame (ORF) encoding the RNA-dependent RNA polymerase (RdRp) (68.95 kDa). The second segment is 1,786 bp in length and also contains a single ORF encoding a hypothetical protein of 35.19 kDa of unknown function. Based on the sequence of its RdRp, BdBMV1 is phylogenetically related to several other unclassified dsRNA mycoviruses, including Cryphonectria parasitica bipartite mycovirus 1 (CpBV1), and has a distant relationship to members of the family Partitiviridae.
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Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384
Xie J, Jiang D (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68
Ghabrial SA, Castón JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479–480:356–368
Koonin EV, Dolja VV, Krupovic M, Varsani A, Wolf YI, Yutin N, Zerbini FM, Kuhn JH (2020) Global organization and proposed megataxonomy of the virus world. Microbiol Mol Biol Rev 84:2
Wang Y, Zhao H, Xue C, Xu C, Geng Y, Zang R, Guo Y, Wu H, Zhang M (2020) Complete genome sequence of a novel mycovirus isolated from the phytopathogenic fungus Corynespora cassiicola in China. Arch Virol 165(10):2401–2404
Liu C, Li M, Redda ET, Mei J, Zhang J, Elena SF, Wu B, Jiang X (2019) Complete nucleotide sequence of a novel mycovirus from Trichoderma harzianum in China. Arch Virol 164(4):1213–1216
Lee SH, Yun SH, Chun J, Kim DH (2017) Characterization of a novel dsRNA mycovirus of Trichoderma atroviride NFCF028. Arch Virol 162(4):1073–1077
Hu Z, Guo J, Da Gao B, Zhong J (2020) A novel mycovirus isolated from the plant-pathogenic fungus Alternaria dianthicola. Arch Virol 165(9):2105–2109
Lin Y, Zhang H, Zhao C, Liu S, Guo L (2015) The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28. Arch Virol 160(2):577–580
Yang D, Wu M, Zhang J, Chen W, Li G, Yang L (2018) Sclerotinia minor endornavirus 1, a novel pathogenicity debilitation-associated mycovirus with a wide spectrum of horizontal transmissibility. Viruses 10:11
Yu X, Li B, Fu Y, Jiang D, Ghabrial SA, Li G, Peng Y, Xie J, Cheng J, Huang J, Yi X (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci USA 107(18):8387–8392
Nuss DL (2005) Hypovirulence: mycoviruses at the fungal-plant interface. Nat Rev Microbiol 3(8):632–642
Heiniger U, Rigling D (1994) Biological control of chestnut blight in Europe. Annu Rev Phytopathol 32(1):581–599
Dissanayake A, Phillips A, Li X, Hyde K (2016) Botryosphaeriaceae: current status of genera and species. Mycosphere 7(7):1001–1073
Marsberg A, Kemler M, Jami F, Nagel JH, Postma-Smidt A, Naidoo S, Wingfield MJ, Crous PW, Spatafora JW, Hesse CN (2017) Botryosphaeria dothidea: a latent pathogen of global importance to woody plant health. Mol Plant Pathol 18(4):477–488
Tang W, Ding Z, Zhou Z, Wang Y, Guo L (2012) Phylogenetic and pathogenic analyses show that the causal agent of apple ring rot in China is Botryosphaeria dothidea. Plant Dis 96(4):486–496
Xu C, Wang C, Ju L, Zhang R, Biggs AR, Tanaka E, Li B, Sun G (2015) Multiple locus genealogies and phenotypic characters reappraise the causal agents of apple ring rot in China. Fungal Divers 71(1):215–231
Zhai L, Zhang M, Lv G, Chen X, Jia N, Hong N, Wang G (2014) Biological and molecular characterization of four Botryosphaeria species isolated from pear plants showing stem wart and stem canker in China. Plant Dis 98(6):716–726
Liu HX, Liang J, Zhao JP et al (2007) Study on the etiology of pomegranate canker diseases. Sci Silvae Sin 04:54–58
Yan J-Y, Xie Y, Zhang W, Wang Y, Liu J-K, Hyde KD, Seem RC, Zhang G-Z, Wang Z-Y, Yao S-W (2013) Species of Botryosphaeriaceae involved in grapevine dieback in China. Fungal Divers 61(1):221–236
Ran L, Zhang M, Shen H (2018) First report of red rot of jujube fruit caused by Botryosphaeria dothidea in China. Plant Dis 102(7):1458
Wang L, Jiang J, Wang Y, Hong N, Zhang F, Xu W, Wang G (2014) Hypovirulence of the phytopathogenic fungus Botryosphaeria dothidea: association with a coinfecting chrysovirus and a partitivirus. J Virol 88(13):7517–7527
Zhai L, Hong N, Zhang M, Wang G (2015) Complete dsRNA sequence of a novel victorivirus isolated from the pear stem wart fungus Botryosphaeria dothidea. Arch Virol 160(2):613–616
Zhai L, Xiang J, Zhang M, Fu M, Yang Z, Hong N, Wang G (2016) Characterization of a novel double-stranded RNA mycovirus conferring hypovirulence from the phytopathogenic fungus Botryosphaeria dothidea. Virology 493:75–85
Yang M, Zhai L, Xiao F, Guo Y, Fu M, Hong N, Wang G (2019) Characterization of a novel victorivirus isolated from the phytopathogenic fungus Botryosphaeria dothidea. Arch Virol 164(6):1609–1617
Zhai L, Yang M, Zhang M, Hong N, Wang G (2019) Characterization of a botybirnavirus conferring hypovirulence in the phytopathogenic fungus Botryosphaeria dothidea. Viruses 11(3):266
Liu W, Hai D, Mu F, Yu X, Zhao Y, He B, Xie J, Jiang D, Liu H (2020) Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Botryosphaeria dothidea. Arch Virol 165(4):1033–1037
Yang M, Zhou X, Zhai L, Xiao F, Hong N, Wang G (2020) Molecular characterization of a novel mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea. Arch Virol 165(7):1667–1670
Liu H, Liu M, Zhu H, Zhong J, Liao X, Zhou Q (2021) Molecular characterization of a novel mitovirus from the plant-pathogenic fungus Botryosphaeria dothidea. Arch Virol 166(2):633–637
Morris T, Dodds J (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69(8):854–858
Xiang J, Fu M, Hong N, Zhai L, Xiao F, Wang G (2017) Characterization of a novel botybirnavirus isolated from a phytopathogenic Alternaria fungus. Arch Virol 162(12):3907–3911
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23(21):2947–2948
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874
Nicholas KB (1997) GeneDoc: analysis and visualization of genetic variation. Embnew news 4:14
Bruenn JA (1993) A closely related group of RNA-dependent RNA polymerases from double-stranded RNA viruses. Nucleic Acids Res 21(24):5667–5669
This research was supported by the Scientific Research Fund of Hunan Provincial Education Department (19K040), the Hunan Provincial Innovation Foundation for Postgraduate (CX20190522), and Excellent Doctoral Dissertation Cultivating Fund of Hunan Agricultural University (YB2019005).
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Liu, H., Wang, H. & Zhou, Q. A novel mycovirus isolated from the plant-pathogenic fungus Botryosphaeria dothidea. Arch Virol 166, 1267–1272 (2021). https://doi.org/10.1007/s00705-021-04983-6