A novel mycovirus isolated from the plant-pathogenic fungus Botryosphaeria dothidea

Abstract

A novel virus, Botryosphaeria dothidea bipartite mycovirus 1 (BdBMV1), was isolated from the plant-pathogenic fungus Botryosphaeria dothidea strain HNDT1, and the complete nucleotide sequence of its genome was determined. BdBMV1 consists of two genomic segments. The first segment is 1,976 bp in length and contains a single open reading frame (ORF) encoding the RNA-dependent RNA polymerase (RdRp) (68.95 kDa). The second segment is 1,786 bp in length and also contains a single ORF encoding a hypothetical protein of 35.19 kDa of unknown function. Based on the sequence of its RdRp, BdBMV1 is phylogenetically related to several other unclassified dsRNA mycoviruses, including Cryphonectria parasitica bipartite mycovirus 1 (CpBV1), and has a distant relationship to members of the family Partitiviridae.

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Acknowledgements

This research was supported by the Scientific Research Fund of Hunan Provincial Education Department (19K040), the Hunan Provincial Innovation Foundation for Postgraduate (CX20190522), and Excellent Doctoral Dissertation Cultivating Fund of Hunan Agricultural University (YB2019005).

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Correspondence to Qian Zhou.

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Liu, H., Wang, H. & Zhou, Q. A novel mycovirus isolated from the plant-pathogenic fungus Botryosphaeria dothidea. Arch Virol 166, 1267–1272 (2021). https://doi.org/10.1007/s00705-021-04983-6

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