Complete genome sequence of aucuba ringspot virus


A new badnavirus, aucuba ringspot virus (AuRV), was identified in plants of Aucuba japonica showing mild mosaic, vein banding, and yellow ringspot symptoms on the leaves. The complete nucleotide sequence of the AuRV genome was determined and found to be 9,092 nt in length, and the virus was found to have a genome organization typical of members of the genus Badnavirus. ORF3 was predicted to encode a polyprotein containing conserved movement protein, coat protein, aspartic protease, reverse transcriptase (RT), and RNase H domains. Phylogenetic analysis suggested that this virus is most closely related to codonopsis vein clearing virus but belongs to a distinct species, based on only 69.6% nucleotide sequence identity within the part of ORF 3 encoding the RT and RNase H domains. The vector of AuRV is unknown, but based on phylogenetic relationships, it is predicted to be a type of aphid.

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We thank NODAI (Tokyo University of Agriculture) Genome Research Center for the sequence of the sample.

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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Ayaka Uke, Marita S. Pinili, Keiko T. Natsuaki, and Andrew D. W. Geering. The first draft of the manuscript was written by Ayaka Uke, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ayaka Uke.

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The aucuba ringspot virus genome sequence has been deposited in the DDBJ/EMBL/GenBank database under accession code LC487411.

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Uke, A., Pinili, M.S., Natsuaki, K.T. et al. Complete genome sequence of aucuba ringspot virus. Arch Virol 166, 1227–1230 (2021).

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