Inhibition of AMP-activated protein kinase in respiratory syncytial virus infection activates lipid metabolism

Archives of Virology volume 166, pages1177–1182(2021)Cite this article

Abstract

Respiratory syncytial virus (RSV) is most commonly associated with upper respiratory tract infections during childhood. The lipid composition of cells and lipogenic enzymes play an important role in RSV infection. There are controversial data about whether lipid biosynthesis regulators such as AMP-activated protein kinase (AMPK) are deregulated by RSV. Hence, we examined whether the activation state of AMPK is altered in RSV-infected HEp-2 cells. Our data show that RSV infection inhibits AMPK activity, favoring the activation of downstream lipogenic effectors and cellular lipid anabolism in HEp-2 cells.

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Acknowledgements

This study was supported by the National Council on Science and Technology CONACYT (CB-2015-01-255007 to M.L.J.) and Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes” (212250-3210-21007-03-15 to M.L.J.) L.D.G.G, T.A.V.P. received a fellowship from CONACYT. Additionally, M.LJ., M.M.C., J.G.C., H.A.R., and A.C.H.R. acknowledge their membership of the National System of Researches (SNI).

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Author notes

  1. Luis Didier González García and Macario Martínez Castillo contributed equally to this work.

Affiliations

  1. Departamento de Inmunobioquímica, Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes”, Montes Urales 800, Col. Lomas Virreyes, CP 11000, Ciudad de México, México

    Luis Didier González-García, Tania Allin Vargas-Pavía, José Manuel Ulloa-Aguilar, Addy Cecilia Helguera-Repetto & Moisés León-Juárez

  2. Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Casco de Santo Tomas, 11340, Ciudad de México, México

    Macario Martínez-Castillo

  3. Laboratorio de Inmunología y Microbiología Molecular, División Académica multidisciplinaria de Jalpa de Méndez, Departamento de Genómica, Universidad Juárez Autónoma de Tabasco, Jalpa de Méndez, México

    Haruki Arévalo-Romero

  4. Laboratorio de Genómica del Metabolismo Óseo, Instituto Nacional de Medicina Genómica, Ciudad de México, México

    Guadalupe Léon-Reyes

  5. Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del I.P.N., Ciudad de México, México

    Julio García-Cordero

Authors
  1. Luis Didier González-García
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  2. Macario Martínez-Castillo
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  3. Tania Allin Vargas-Pavía
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  4. José Manuel Ulloa-Aguilar
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  5. Haruki Arévalo-Romero
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  6. Guadalupe Léon-Reyes
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  7. Addy Cecilia Helguera-Repetto
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  8. Julio García-Cordero
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  9. Moisés León-Juárez
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Contributions

Conceived and designed the experiments: LDGG, MMC, MLJ. Performed the experiments: LDGG, MMC, TAVP, JMUA. Contributed reagents/materials/analysis tools: HAR, ACHR, GLR. Wrote the paper: MLJ and MMC. Coordinated and facilitated the project: MLJ. All authors have read and approved the final manuscript.

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Correspondence to Moisés León-Juárez.

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González-García, L.D., Martínez-Castillo, M., Vargas-Pavía, T.A. et al. Inhibition of AMP-activated protein kinase in respiratory syncytial virus infection activates lipid metabolism. Arch Virol 166, 1177–1182 (2021). https://doi.org/10.1007/s00705-021-04974-7

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