Inhibition of AMP-activated protein kinase in respiratory syncytial virus infection activates lipid metabolism

Abstract

Respiratory syncytial virus (RSV) is most commonly associated with upper respiratory tract infections during childhood. The lipid composition of cells and lipogenic enzymes play an important role in RSV infection. There are controversial data about whether lipid biosynthesis regulators such as AMP-activated protein kinase (AMPK) are deregulated by RSV. Hence, we examined whether the activation state of AMPK is altered in RSV-infected HEp-2 cells. Our data show that RSV infection inhibits AMPK activity, favoring the activation of downstream lipogenic effectors and cellular lipid anabolism in HEp-2 cells.

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Acknowledgements

This study was supported by the National Council on Science and Technology CONACYT (CB-2015-01-255007 to M.L.J.) and Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes” (212250-3210-21007-03-15 to M.L.J.) L.D.G.G, T.A.V.P. received a fellowship from CONACYT. Additionally, M.LJ., M.M.C., J.G.C., H.A.R., and A.C.H.R. acknowledge their membership of the National System of Researches (SNI).

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Conceived and designed the experiments: LDGG, MMC, MLJ. Performed the experiments: LDGG, MMC, TAVP, JMUA. Contributed reagents/materials/analysis tools: HAR, ACHR, GLR. Wrote the paper: MLJ and MMC. Coordinated and facilitated the project: MLJ. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Moisés León-Juárez.

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González-García, L.D., Martínez-Castillo, M., Vargas-Pavía, T.A. et al. Inhibition of AMP-activated protein kinase in respiratory syncytial virus infection activates lipid metabolism. Arch Virol 166, 1177–1182 (2021). https://doi.org/10.1007/s00705-021-04974-7

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