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Complete genome sequence of ornithogalum virus 3

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Abstract

Ornithogalum thyrsoides, a widely cultivated bulbous ornamental plant endemic to South Africa, has significant commercial value as a pot plant and for the production of cut flowers. However, infection by viruses threatens the success of commercial cultivation, as symptoms negatively affect the appearance of the plant and flowers. To date, four Ornithogalum-infecting viruses have been reported. Complete genome sequence data are available for three of these viruses, but the genome of the potyvirus ornithogalum virus 3 (OV3) has not been fully sequenced. In this study, the complete sequence of OV3 was determined by high-throughput sequencing (HTS) and validated by Sanger sequencing. Based on recognition of protease cleavage patterns and multiple sequence alignments with closely related viruses, the polyprotein of OV3 was predicted to be proteolytically cleaved to produce 10 mature peptides containing domains conserved in members of the genus Potyvirus. Phylogenetic analysis and species demarcation criteria confirm the previous classification of OV3 as a member of a separate species in this genus. This is the first report of a complete genome sequence of OV3.

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Author information

Authors and Affiliations

  1. Department of Genetics, Stellenbosch University, Stellenbosch, South Africa

    I. Mostert, R. Bester, D. Aldrich, M. Visser, H. J. Maree & J. T. Burger

  2. Citrus Research International, Stellenbosch, South Africa

    H. J. Maree

  3. Agricultural Research Council-Roodeplaat Vegetable and Ornamental Plants, Pretoria, South Africa

    I. Gazendam & M. Cloete

Authors
  1. I. Mostert
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  2. R. Bester
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  3. D. Aldrich
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  4. M. Visser
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  5. I. Gazendam
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  6. M. Cloete
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  7. H. J. Maree
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  8. J. T. Burger
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Rachelle Bester, Ilani Mostert, Dirk Aldrich, and Marike Visser. The first draft of the manuscript was written by Ilani Mostert, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to H. J. Maree.

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All authors declare they have no conflict of interest.

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This article does not contain any studies involving human participants or animals.

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Handling Editor: Ioannis E. Tzanetakis.

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Mostert, I., Bester, R., Aldrich, D. et al. Complete genome sequence of ornithogalum virus 3. Arch Virol 166, 1213–1216 (2021). https://doi.org/10.1007/s00705-021-04965-8

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  • DOI: https://doi.org/10.1007/s00705-021-04965-8

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